GUO Yan-rong, FU Cui-li. Simultaneously Determination of Matrine and Stilbene Glucoside in Renzaozhiyang Capsule by SPE-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(12): 75-77.
GUO Yan-rong, FU Cui-li. Simultaneously Determination of Matrine and Stilbene Glucoside in Renzaozhiyang Capsule by SPE-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(12): 75-77. DOI: 10.13422/j.cnki.syfjx.2010.12.024.
Simultaneously Determination of Matrine and Stilbene Glucoside in Renzaozhiyang Capsule by SPE-HPLC
摘要
目的:建立润燥止痒胶囊中苦参碱和二苯乙烯苷的含量测定方法。方法:采用固相萃取-HPLC
色谱柱为XTerraRP18(3.9 mm×150 mm
5μm)
流速1.0 mL·min-1
乙腈-水(20∶80)为流动相
检测波长为320 nm。结果:苦参碱的线性范围为0.302 6~1.513 g·L-1
r=0.999 7
平均回收率为98.65%
RSD 1.90%(n=6);二苯乙烯苷的线性范围为1.048~8.384 mg·L-1
r=0.999 5
平均回收率为97.45%
RSD 1.89%(n=6)。结论:方法简便、快速、准确
适合该胶囊中苦参碱和二苯乙烯苷的含量测定。
Abstract
Objective: To establish a method for the determination of matrine and stilbene glucoside in Renzao Zhiyang Capsule.Method: SPE-HPLC was conducted on a XTerra RP18 column(3.9 mm × 150 mm
5 μm) with acetonitrile-water(20∶80)
flow rate 1.0 mL·min-1
detecting at 320 nm.Result: Matrine was linear in the range of 0.302 6-1.513 g·L-1(r=0.999 7)
the average recovery was 98.65%
RSD 1.90%(n=6).Stilbene glucoside was linear in the range of 1.048-8.384 mg·L-1(r = 0.999 5)
the average recovery was 97.45%
RSD 1.89%(n=6).Conclusion: The method was simple
sensitive
accurate and suitable for the determination of matrine and stilbene glucoside in Renzao Zhiyang Capsule.
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Related Author
GUO Yan-rong
FU Cui-li
CHEN Linzhen
ZHANG Xue
CHEN Qi
YU Xue
DAI Shengyun
MA Zhiqiang
Related Institution
Beijing Key Laboratory of Traditional Chinese Medicine(TCM) Quality Evaluation, Beijing University of Chinese Medicine
School of TCM, Beijing University of Chinese Medicine
National Institutes for Food and Drug Control
National Engineering Laboratory for Quality Control Technology of Chinese Materia Medica, Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences