ZHANG Yong, LIAO Qing-chuan, QIU Jin-chun, et al. Simultaneous Determination of Baicalin and Arctiin in Huxitong Oral Solution by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(12): 25-27.
ZHANG Yong, LIAO Qing-chuan, QIU Jin-chun, et al. Simultaneous Determination of Baicalin and Arctiin in Huxitong Oral Solution by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(12): 25-27. DOI: 10.13422/j.cnki.syfjx.2010.12.054.
Simultaneous Determination of Baicalin and Arctiin in Huxitong Oral Solution by HPLC
摘要
目的:建立同时测定呼吸通口服液中有效成分黄芩苷和牛蒡子苷含量的方法。方法:采用高效液相色谱法
色谱柱platisil ODS(4.6 mm×250 mm
5μm)
流动相乙腈-0.02%磷酸溶液(33∶66)
检测波长208 nm
柱温室温。结果:黄芩苷在10~100 mg·L-1
牛蒡子苷在5~50 mg·L-1有良好的线性关系;黄芩苷的平均回收率99.8%
RSD 0.19%;牛蒡子苷的平均回收率100.1%
RSD 0.18%。结论:本方法简便可行
重复性好
可用于呼吸通口服液中黄芩苷和牛蒡子苷的含量测定和质量控制。
Abstract
Objective: To develop an HPLC method for the determination of baicalin and arctiin in Huxitong Oral Solution.Method: Analysis was carried out at room temperature on a platisil ODS column(4.6 mm × 250 mm
5 μm) with acetonitrile-0.02% phosphoric acid as the mobile phase
detecting at 208 nm·Result: The average recovery of baicalin was 99.0%
RSD 0.94% and the linear range was 10-100 mg·L-1
r = 0.999 5;The average recovery of arctiin was 100.5%
RSD 1.29% and the linear range was 5-50 mg·L-1
r = 0.999 7.Conclusion: The method is simple and reproducible.It can be used for the quality control of Huxitong Oral Solution.