Fingerprint Analysis of Curcuma wenyujin

FANG Li1; ZHU Ming2; ZHENG Cheng2; ZHANG Peng1

doi:10.13422/j.cnki.syfjx.2010.12.058

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Fingerprint Analysis of Curcuma wenyujin

更新时间：2024-09-23

- Fingerprint Analysis of Curcuma wenyujin
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 12, Pages: 37-40(2010)
- 作者机构：
  
  1. 浙江中医药大学药学院
  2. 浙江省食品药品检验所
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2010.12.058
  CLC： R284.1
- Published：2010
- 稿件说明：
移动端阅览
[1]方丽,祝明,郑成,张鹏.温莪术药材指纹图谱的研究[J].中国实验方剂学杂志,2010,16(12):37-40.

FANG Li1, ZHU Ming2, ZHENG Cheng2, et al. Fingerprint Analysis of Curcuma wenyujin[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(12): 37-40.
[1]方丽,祝明,郑成,张鹏.温莪术药材指纹图谱的研究[J].中国实验方剂学杂志,2010,16(12):37-40. DOI： 10.13422/j.cnki.syfjx.2010.12.058.

FANG Li1, ZHU Ming2, ZHENG Cheng2, et al. Fingerprint Analysis of Curcuma wenyujin[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(12): 37-40. DOI： 10.13422/j.cnki.syfjx.2010.12.058.

摘要

目的:建立温莪术的HPLC指纹图谱分析色谱条件

拟定温莪术指纹特征图谱的共有模式。方法:采用kromasilC18色谱柱（4.6 mm×250 mm

5μm）;流动相为乙腈-0.1%磷酸（梯度洗脱）;检测波长为216 nm;柱温为30℃;流速为1mL·min-1。结果:温莪术共标示出13个共有峰。10批温莪术相似度较好

均大于0.95。用本文建立的测定方法

能够明确区别温莪术和广西莪术及蓬莪术。结论:方法准确可靠

重复性好

为温莪术质量控制提供了保证。

Abstract

Objective: To establish a method of fingerprint analysis of Curcuma wenyujin by HPLC.Method: HPLC was performed on a kromasil ODS C18 analytical column（4.6 mm×250 mm

5 μm） with gradient elution（0-45 min

45% A→95% A;45-55 min

95% A） of acetonitrile（A）-0.1% phosphoric acid（B） at the flow rate of 1.0 mL·min-1.The column temperature was 30 ℃ and UV detection wavelength was set at 216 nm.Result: Thirteen peaks were identified as the characteristic fingerprints of C.wenyujin and the fingerprints profiles of the 10 samples from the same species C.wenyujin presented high similarities.The similarities of different species of Curcuma were low.The fingerprint profile was able to identify different species of Curcuma.Conclusion: This method is rapid

precise and replicable

which is helpful to control the quality of Curcumae.

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Related Author

FANG Li

ZHU Ming

ZHENG Cheng

ZHANG Peng

Hong-xing MA

Long-fei LIN

Yu-ling LIU

Sai FU

Related Institution

Jilin Agricultural University

Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences

Chengde Medical University

Jilin Huakang Pharmaceutical Co. Ltd.

1),WANG De-sheng~1,WANG Jian-xiu~1,DUAN Shu-rong~1,LI Wen-lan~2,BAI Jing~2

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