WEI Xiang-zhong, YUE Li, QIN Song-mei, et al. Determination of Germacrone in Superfine Powder of Different Processed Products of Curcuma kwangsiensis by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(12): 45-47.
WEI Xiang-zhong, YUE Li, QIN Song-mei, et al. Determination of Germacrone in Superfine Powder of Different Processed Products of Curcuma kwangsiensis by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(12): 45-47. DOI: 10.13422/j.cnki.syfjx.2010.12.060.
Determination of Germacrone in Superfine Powder of Different Processed Products of Curcuma kwangsiensis by HPLC
Objective: To determine the content of germacrone from different processed products of Curcuma kwangsiensis before and after the superfine comminution.Method: With the ball milling technology at low temperatures(-30 ± 2) ℃
the size of the superfine powder was between 1.0-2.0 μm.The HPLC analysis was performed on Hypersil ODS2 C18(4.6 mm × 250 mm
5 μm)
the mobile phase was acetonitrile-water(65∶ 35)
the detection wavelength was at 210 nm.Result: The germacrone content of commom powder(75-53 μm) was higher than the content of the superfine powder(1.0-2.0 μm).Conclusion: The size of superfine powder influenced the content of germacrone of the four different processed products of C.kwangsiensis evidently
and this offered referenced data for the different processed products of C.kwangsiensis.