Determination of Ergosterol in Lingzhi Dispensing Granules

CHEN Yong1; ZENG Ping1; WEN Qing-wei2; HU Feng1; YANG Chen1; LIU Ying-li1; WU Shi-hong1

doi:10.13422/j.cnki.syfjx.2010.12.063

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Determination of Ergosterol in Lingzhi Dispensing Granules

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- Determination of Ergosterol in Lingzhi Dispensing Granules
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 12, Pages: 53-55(2010)
- 作者机构：
  
  1. 广西中医学院
  2. 培力(南宁)药业有限公司
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2010.12.063
  CLC： R286.0
- Published：2010
- 稿件说明：
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[1]陈勇,曾萍,温庆伟,胡丰,杨晨,刘瑛丽,巫世红.灵芝配方颗粒中麦角甾醇的含量测定[J].中国实验方剂学杂志,2010,16(12):53-55.

CHEN Yong1, ZENG Ping1, WEN Qing-wei2, et al. Determination of Ergosterol in Lingzhi Dispensing Granules[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(12): 53-55.
[1]陈勇,曾萍,温庆伟,胡丰,杨晨,刘瑛丽,巫世红.灵芝配方颗粒中麦角甾醇的含量测定[J].中国实验方剂学杂志,2010,16(12):53-55. DOI： 10.13422/j.cnki.syfjx.2010.12.063.

CHEN Yong1, ZENG Ping1, WEN Qing-wei2, et al. Determination of Ergosterol in Lingzhi Dispensing Granules[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(12): 53-55. DOI： 10.13422/j.cnki.syfjx.2010.12.063.

摘要

目的:建立灵芝配方颗粒中麦角甾醇的含量测定方法。方法:反相HPLC

流动为甲醇-水（99∶1）

检测波长为281 nm

流速1.2 mL·min-1

柱温25℃。结果:麦角甾醇对照品进样量在0.223 7～1.342μg呈良好线性关系

回归方程为Y=1 104.468X+1.168

r=0.999 9

回收率为103.1%

RSD为0.48%（n=6）。结论:本法简便易行

重复性好

可作为灵芝配方颗粒的质量控制方法。

Abstract

Objective: To establish a quantitative method for determination of ergosterol in Lingzhi Dispensing Granule.Method: A RP-HPLC method was used to determine the content of ergosterol with methanol-water（99∶ 1） as the mobile phase and detection wavelength was 281 nm

The flow rate was 1.2 mL.min-1 and the column temperature was 25 ℃.Result: The linearity of ergosterol were good in range of 0.223 7～1.342 μg

and the regression equation was Y=1 104.468 X + 1.168（r=0.999 9）.The average recovery was 103.1% and the RSD value of repeatability was 0.48%（n=6）.Conclusion: This method is simple and reproducible

and it can be used for quality control of Lingzhi Dispensing Granule.

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Related Author

WU Shi-hong

LIU Ying-li

YANG Chen

HU Feng

WEN Qing-wei

ZENG Ping

CHEN Yong

Cheng-zan ZHONG

Related Institution

Key Laboratory of Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences

Jiangxi Guoyao Co. Ltd.

Ethnic Pharmaceutical Institute of Southwest University for Nationalities

School of Life Sciences,Guizhou Normal University

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