Determination of Berberine Hydrochloride in Smilax Glabra Roxby Capsules by RP-HPLC

MENG Yan1; NI Jian2; JIANG Lin1

doi:10.13422/j.cnki.syfjx.2010.12.064

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Determination of Berberine Hydrochloride in Smilax Glabra Roxby Capsules by RP-HPLC

更新时间：2024-09-23

- Determination of Berberine Hydrochloride in Smilax Glabra Roxby Capsules by RP-HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 12, Pages: 59-61(2010)
- 作者机构：
  
  1. 新疆医科大学附属中医医院
  2. 北京中医药大学中药学院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2010.12.064
  CLC： R286.0
- Published：2010
- 稿件说明：
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[1]孟艳,倪健,姜林.RP-HPLC测定土茯苓胶囊中盐酸小檗碱的含量[J].中国实验方剂学杂志,2010,16(12):59-61.

MENG Yan1, NI Jian2, JIANG Lin1. Determination of Berberine Hydrochloride in Smilax Glabra Roxby Capsules by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(12): 59-61.
[1]孟艳,倪健,姜林.RP-HPLC测定土茯苓胶囊中盐酸小檗碱的含量[J].中国实验方剂学杂志,2010,16(12):59-61. DOI： 10.13422/j.cnki.syfjx.2010.12.064.

MENG Yan1, NI Jian2, JIANG Lin1. Determination of Berberine Hydrochloride in Smilax Glabra Roxby Capsules by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(12): 59-61. DOI： 10.13422/j.cnki.syfjx.2010.12.064.

摘要

目的:采用RP-HPLC（反相液相色谱法）测定土茯苓胶囊中盐酸小檗碱的含量。方法:色谱柱Kromasil C18柱（4.6 mm×250 mm

5μm）;流动相乙腈-磷酸盐缓冲液[0.43%磷酸二氢钠和0.17%十二烷基磺酸钠

并用三乙胺调pH至7.5]（47∶53）;流速1.0 mL·min-1;检测波长265 nm。结果:盐酸小檗碱在0.042 2～0.527 5μg线性关系良好（r=0.999 1）

平均回收率为101.35%

RSD为1.06%。结论:该法快捷、简便、准确

能更好的控制其质量。

Abstract

Objective: RP-HPLC method was established for determination of berberine hy-dro chloride in Smilax glabra Roxby capsules.Method: With acetonitrile-phosphate as the buffer [0.43% natrium dihydrogen phosphate and 0.17% sodium dodecylsulphate

consisting of pH was adjusted to 7.5 with diethylamide]（47∶ 53） as the mobile

determination wavelength was 265 nm.Result: The linearity was obtained over the range of 0.042 2-0.527 5 μg（r=0.999 1） for the ber-berine hydrochloride.The average recovery of the berberine hydrochloride was 101.35% with RSD of 1.06%.Conclusion: The method was simple

rapid

and accurate as the quality standard.

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Related Author

MENG Yan

Ni Jian

JIANG Lin

Yukun ZHENG

Guangzhong WANG

Xiaowei WU

Qipan JIAN

Yuhang SHU

Related Institution

Pharmacy Faculty， Hubei University of Chinese Medicine

Hubei Engineering Technology Research Center of Chinese Material Medical Processing

Tongji Hospital， Tongji Medical College of Huazhong University of Science and Technology

College of Pharmacy, School of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine

Tibetan Medical College, Qinghai University

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