LIU En-li1, ZHANG Su-qiong1, LIANG Tai-gang1, et al. Determination of Content of Kaempferol in Fuyang Capsule by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(12): 62-63.
LIU En-li1, ZHANG Su-qiong1, LIANG Tai-gang1, et al. Determination of Content of Kaempferol in Fuyang Capsule by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(12): 62-63. DOI: 10.13422/j.cnki.syfjx.2010.12.065.
Determination of Content of Kaempferol in Fuyang Capsule by HPLC
摘要
目的:建立测定肤痒胶囊中山奈素含量的HPLC。方法:用HPLC测定肤痒胶囊中山奈素的含量
采用HypersilODS色谱柱
以甲醇-0.4%磷酸溶液(50∶50)为流动相
流速1.0 mL.min-1
检测波长367 nm
柱温30℃。结果:山奈素含量在27.0~135.0 ng呈良好的线性关系
回收率为100.41%
RSD 1.4%(n=6)。结论:该方法简便
重复性好
可作为肤痒胶囊及其原料中山奈素的含量检测方法。
Abstract
Objective: To determine the content of kaempferol in Fuyang capsule by HPLC.Method: A simple HPLC method was established for the determination of Kaempferol.The mobile phase was methanol-0.4% phosphoric acid(50∶ 50).UV detecting wavelength was at 367 nm.Result: Kaempferol showed a linear relationship in the range of 27.0 ~ 135.0 ng(r = 0.999 9).The average recovery was 104.3% and RSD 2.6%(n = 6).Conclusion: The method can be used for quantitative determination of kaempferol in Fuyang capsule and crude material.