Objective: To investigate the objectivity and validity of animal model for deficiency of kidney yin /yang in traditional Chinese medicine by means of zoogenic thermal activity.Method: Deficiency of kidney yin model and deficiency of kidney yang model in mice were duplicated by subcutaneous injection of hydrocortisone at the dose of 50 mg.kg-1 for 5 d or 25 mg.kg-1 for 10 d

respectively.The remaining rate of animals in different temperature zone were recorded by the hot & cold plate instrument;simultaneously

the drink and food taking

the oxygen consumption and activities of adenosine triphosphatase（ATPase） and SOD

and cAMP /cGMP were detected

so as to investigate the relationship of physical status with energy metabolism /compensation from environment.Result: The remaining rate in hot plate of kidney-yin deficiency model was decreased notably（P < 0.05） in the first 5 days

and then return to normal condition in the last 5 days

and their capability of energy metabolism was decreased significantly（P < 0.05）;while the remaining rate in hot plate of kidney-yang deficiency model mice was decreased in the first 3 days and then increased significantly（P < 0.05 or P < 0.01）

and their capability of energy metabolism was increased significantly（P < 0.05）.The result was consistent with the previous reports in literatures.Conclusion: The method of animal differential distribution on hold & cold plate could show the difference among cold /heat physical model animals

could be used as a novel mean for evaluation animal models of symptoms in traditional Chinese medicine and the nature of traditional Chinese drugs.