Studies on Optimum Ethanol Extraction Process and Antioxidant Activities in vitro of Scutellaria barbata

XUE Hai-ping; ZHU Chun-yun; RUI Xin-yi; ZHANG Ting

doi:10.13422/j.cnki.syfjx.2010.14.022

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Studies on Optimum Ethanol Extraction Process and Antioxidant Activities in vitro of Scutellaria barbata

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- Studies on Optimum Ethanol Extraction Process and Antioxidant Activities in vitro of Scutellaria barbata
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 14, Pages: 12-16(2010)
- 作者机构：
  
  上海中医药大学
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2010.14.022
  CLC： R285;R284.1
- Published：2010
- 稿件说明：
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[1]薛海萍,朱春赟,芮欣忆,张婷.半枝莲醇提工艺的优化及体外抗氧化活性评价的研究[J].中国实验方剂学杂志,2010,16(14):12-16.

XUE Hai-ping, ZHU Chun-yun, RUI Xin-yi, et al. Studies on Optimum Ethanol Extraction Process and Antioxidant Activities in vitro of Scutellaria barbata[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(14): 12-16.
[1]薛海萍,朱春赟,芮欣忆,张婷.半枝莲醇提工艺的优化及体外抗氧化活性评价的研究[J].中国实验方剂学杂志,2010,16(14):12-16. DOI： 10.13422/j.cnki.syfjx.2010.14.022.

XUE Hai-ping, ZHU Chun-yun, RUI Xin-yi, et al. Studies on Optimum Ethanol Extraction Process and Antioxidant Activities in vitro of Scutellaria barbata[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(14): 12-16. DOI： 10.13422/j.cnki.syfjx.2010.14.022.

摘要

目的:用正交试验法对半枝莲的乙醇提取工艺进行优化研究

通过体外试验对提取物的抗氧化活性进行评价。方法:以浸膏得率、总黄酮和野黄芩苷从生药中的提取率为评价指标

选择乙醇体积分数（%）、乙醇用量（倍）、提取时间（h）和提取次数（次）为考察因素

利用正交试验L9（34）确定半枝莲的最佳醇提工艺。基于氧化还原反应原理的FRAP法用于总抗氧化能力的评估

DPPH法用于评价自由基清除能力。结果:最佳工艺条件为用药材10倍量的75%乙醇加热回流提取3次

每次1 h。半枝莲提取物以浓度依赖关系发挥着很好的抗氧化作用

对DPPH自由基具有较强的清除和抑制作用。结论:该提取工艺具有较佳的重复性和可行性。半枝莲醇提物显示了较好的抗氧化活性

值得进一步开发研究。

Abstract

Objective:To study the optimum ethanol extraction process and antioxidant activities in vitro of Scutellaria barbata D.Don.Method:The optimum ethanol extraction process was established with the orthogonal design.The extraction rate of extractum

total flavonoids and scutellarin from crude material were chosen as the as-sessment guideline.Antioxidant activities in vitro of the extract were evaluated by FRAP assay and DPPH assay compared with Trolox.Result:The optimum ethanol extraction process conditions were as follows:10 fold of 75% ethanol was added

to be extracted 3 times by reflux and 1 h per time.The extract of S.barbata exerted antioxidant role with dose-dependent relation in the two systems through oxidation-reduction reaction and inhibition of free radi-cal.Conclusion:Validation studies proved that the ethanol extraction process had good reproducibility and feasibil-ity.The ethanol extract of S.barbata exhibited a strong antioxidant activity in vitro

which is worthy of further stud-ies.

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ZHANG Ting

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Related Institution

Bio-Engineering College of Xihua University

Second Affiliated Hospital of Zhengzhou University

Pharmacy Technology Center，Tsinghua University

Center of Pharmacy R&D，XinJiang Huachun Bio-Pharmaceutical Co. Ltd.

School of Life Sciences， Tsinghua University

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