Study on Purification Process of Polysaccharides from Sugarcane Biogases

WANG Xu-cong; LIU Li; ZHANG Lu; WENG Li-dong; SUN Xue-gang; LIU Qiang

doi:10.13422/j.cnki.syfjx.2010.14.027

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Study on Purification Process of Polysaccharides from Sugarcane Biogases

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- Study on Purification Process of Polysaccharides from Sugarcane Biogases
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 14, Pages: 31-34(2010)
- 作者机构：
  
  南方医科大学中医药学院中药制剂学教研室
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2010.14.027
  CLC： R284.1
- Published：2010
- 稿件说明：
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[1]王许聪,刘莉,张璐,翁立冬,孙学刚,刘强.甘蔗渣多糖的纯化工艺[J].中国实验方剂学杂志,2010,16(14):31-34.

WANG Xu-cong, LIU Li, ZHANG Lu, et al. Study on Purification Process of Polysaccharides from Sugarcane Biogases[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(14): 31-34.
[1]王许聪,刘莉,张璐,翁立冬,孙学刚,刘强.甘蔗渣多糖的纯化工艺[J].中国实验方剂学杂志,2010,16(14):31-34. DOI： 10.13422/j.cnki.syfjx.2010.14.027.

WANG Xu-cong, LIU Li, ZHANG Lu, et al. Study on Purification Process of Polysaccharides from Sugarcane Biogases[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(14): 31-34. DOI： 10.13422/j.cnki.syfjx.2010.14.027.

摘要

目的:研究甘蔗渣中多糖的最佳纯化工艺。方法:用Savage法、TCA法脱蛋白;用双氧水法、活性炭法脱色;用AB-8

DB301

D101

MG-1大孔树脂纯化甘蔗渣多糖

用苯酚-硫酸法测定多糖的含量。结果:最佳工艺为用Savage法脱蛋白、活性炭脱色、MG-1型大孔吸附树脂纯化（上样量为0.3 g

柱高35 cm、流速10 mL/15 min）

经富集后甘蔗渣多糖的含量由51.11%提高到了83.57%。结论:本实验为甘蔗渣多糖的分离纯化条件提供了参考

为甘蔗渣的进一步开发研究提供了依据。

Abstract

Objective:To explore the optimal purification process of the polysaccharides from sugarcane biogases.Method:To explore the optimum technology

we compared Savage method with TCA method for depro-teinization and hydrogen peroxide solution method with active carbon method for decoloration.And four common macroporous absorption resins were compared for differences in adsorption and desorption performance.Then phe-nol-sulfuric acid method was used to determine the content of the polysaccharides from sugarcane biogases.Result:The optimum purification technological conditions were determined as following:deproteinization with savage meth-od;discoloration with active carbon method;purification with macroporous absorption resins of MG-1（the upper sample weight is 0.3 g;the column high is 35 cm and the flow rate is 10 mL /15 min）.The polysaccharides of sug-arcane biogases improved from 51.11% to 83.57%.Conclusion:This experiment provided a reference for the pur-ification of polysaccharides from sugarcane biogases and provided an evidence for the further development of sugar-cane biogases.

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Related Author

WANG Xu-cong

LIU Li

ZHANG Lu

WENG Li-dong

SUN Xue-gang

LIU Qiang

Bing-bing ZHANG

Ze-min OU

Related Institution

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

School of Pharmacy， Jiangxi University of Chinese Medicine

School of Pharmacy，Tianjin University of Traditional Chinese Medicine

Ethnic Medicine School, Chengdu University of Traditional Chinese Medicine

Tibetan Medical College, Qinghai University

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