Content Determination of Arctiin and Arctigen from Different Parts of Arctium tomentosum by RP-HPLC

GE Liang1; ZHANG Hao-ke1; TIAN Shu-ge1

doi:10.13422/j.cnki.syfjx.2010.14.030

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Content Determination of Arctiin and Arctigen from Different Parts of Arctium tomentosum by RP-HPLC

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- Content Determination of Arctiin and Arctigen from Different Parts of Arctium tomentosum by RP-HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 14, Pages: 41-43(2010)
- 作者机构：
  
  1. 新疆名医名方与特色方剂学重点实验室
  2. 新疆医科大学中医学院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2010.14.030
  CLC： R284.1
- Published：2010
- 稿件说明：
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[1]葛亮,张浩科,田树革.反相液相色谱法测定毛头牛蒡不同部位中牛蒡苷和牛蒡苷元的含量[J].中国实验方剂学杂志,2010,16(14):41-43.

GE Liang1, ZHANG Hao-ke1, TIAN Shu-ge1. Content Determination of Arctiin and Arctigen from Different Parts of Arctium tomentosum by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(14): 41-43.
[1]葛亮,张浩科,田树革.反相液相色谱法测定毛头牛蒡不同部位中牛蒡苷和牛蒡苷元的含量[J].中国实验方剂学杂志,2010,16(14):41-43. DOI： 10.13422/j.cnki.syfjx.2010.14.030.

GE Liang1, ZHANG Hao-ke1, TIAN Shu-ge1. Content Determination of Arctiin and Arctigen from Different Parts of Arctium tomentosum by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(14): 41-43. DOI： 10.13422/j.cnki.syfjx.2010.14.030.

摘要

目的:对毛头牛蒡不同部位中牛蒡苷和牛蒡苷元进行含量测定。方法:采用反相液相色谱法

Kromasil C18柱（4.6 mm×250 mm

5μm）

流动相甲醇-水（55∶45）

检测波长280 nm

流速1.0 mL.min-1。结果:毛头牛蒡根中含有少量牛蒡苷

不含牛蒡苷元;茎、叶柄中几乎不含牛蒡苷及牛蒡苷元;叶、花序中不含牛蒡苷而含有少量的牛蒡苷元;而其种子中二者含量相对较高。结论:该方法简便、准确

可同时测定毛头牛蒡中牛蒡苷和牛蒡苷元的含量

为更好的开发利用毛头牛蒡提供科学依据。

Abstract

Objective:To establish an HPLC method for determination of different parts of Arctium tomento-sum.Method:Chromatographic separation was achieved by using RP-HPLC system consisting of a Shimadzu LC-6AD and Kromasil C18column（4.6 mm × 250 mm

5 μm）.The mobile phase was consisted of methanol and water（55∶ 45）.Detection wavelength was at 280 nm.The flow rate was set at 1.0 mL.min-1.Result:A small amount of arctiin and no arctigenin was found in the root;while stem and leaf nearly did not contain.Leaves and inflores-cence did not contain arctiin and a few arctigenin.But both ingredients were higher in the fruit than in the leaves and inflorescence.Conclusion:The method was simple and accurate

and it could be used for simultaneous deter-mination of arctiin and arctigenin from Radix Arctium

to provide the basis for a better development of A.tomento-sum Mill.

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Related Author

TIAN Shu-ge

ZHANG Hao-ke

GE Liang

GAO Sai-nan

DU Wen-wen

LI Chun-xiang

JIANG Lei

LI Sen

Related Institution

The Traditional Chinese Medical Hospital Affiliated to Xinjiang Medical University

The Traditional Chinese Medical of Beijing University of Chinese Medicine

Nanjing Children’s Hospital,Nanjing Medical University

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