Simultaneous Determination of Three Active Constitutents in Erchen Tang by High Performance Capillary Electrophoresis

ZHANG Chao-yun1; HUANG Xian-zhang1; GAO Yan-ming2

doi:10.13422/j.cnki.syfjx.2010.14.031

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Simultaneous Determination of Three Active Constitutents in Erchen Tang by High Performance Capillary Electrophoresis

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- Simultaneous Determination of Three Active Constitutents in Erchen Tang by High Performance Capillary Electrophoresis
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 14, Pages: 48-50(2010)
- 作者机构：
  
  1. 南阳理工学院
  2. 贵阳中医学院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2010.14.031
  CLC： R284.1
- Published：2010
- 稿件说明：
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[1]张超云,黄显章,高言明.高效毛细管电泳法同时测定二陈汤中3种成分含量[J].中国实验方剂学杂志,2010,16(14):48-50.

ZHANG Chao-yun1, HUANG Xian-zhang1, GAO Yan-ming2. Simultaneous Determination of Three Active Constitutents in Erchen Tang by High Performance Capillary Electrophoresis[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(14): 48-50.
[1]张超云,黄显章,高言明.高效毛细管电泳法同时测定二陈汤中3种成分含量[J].中国实验方剂学杂志,2010,16(14):48-50. DOI： 10.13422/j.cnki.syfjx.2010.14.031.

ZHANG Chao-yun1, HUANG Xian-zhang1, GAO Yan-ming2. Simultaneous Determination of Three Active Constitutents in Erchen Tang by High Performance Capillary Electrophoresis[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(14): 48-50. DOI： 10.13422/j.cnki.syfjx.2010.14.031.

摘要

目的:建立二陈汤中橙皮苷、甘草酸和甘草次酸的高效毛细管电泳（HPCE）含量测定方法。方法:采用高效毛细管电泳法

电泳条件为未涂层石英毛细管柱50μm×95 cm（有效长度86.5 cm）

运行缓冲液50 mmol.L-1硼砂溶液（含10%乙腈

pH 9.28）;压力进样50 kPa×20 s

电压为24 kV

柱温20℃

检测波长254 nm。结果:橙皮苷、甘草酸与甘草次酸分别在0.10～2.40 g.L-1

0.04～0.72 g.L-1

0.01～0.18 g.L-1呈良好线性关系

r分别为0.999 4

0.999 4

0.999 6

平均回收率分别为98.17%

98.72%

98.48%

RSD分别为1.49%

2.95%

2.25%。结论:该方法简便、快速、准确

在一定程度上弥补了高效液相法的不足

为二陈汤电泳指纹图谱的建立提供了依据

并为该方药效物质基础的研究奠定了基础。

Abstract

Objective:To establish a high performance capillary electrophoresis（HPCE） method for the de-termination of hesperidin

glycyrrhizic acid and glycyrrhetinic acid in Erchen Tang.Method:Fused silica capillary coated（50 μm × 95 cm） with detection window at 86.5 cm from anode was employed and 50 mmol.L-1 borax solu-tion（10% acetonitrile

pH 9.28） served as the running buffer.Other conditions were as follows:electrokinetic in-jection 20 s.analytical voltage 24 kV

detection wavelength 254 nm and temperature 20 ℃.Result:The linearity of hesperidin was in the range of 0.10-2.40 g.L-1（r = 0.999 4）

the average recovery was 98.17% and RSD1.49%.The linearity of glycyrrhizic acid was in the range of 0.04-0.72 g.L-1（r = 0.999 4）

the average recovery was 98.72% and RSD 2.95%.The linearity of glycyrrhetinic acid was in the range of 0.01-0.18 g.L-1（r = 0.999 6）

the average recovery was 98.48% and RSD 2.25%.Conclusion:The method is rapid

simple and accurate and it provides the basis of the fingerprints of CE and composite herbal medicines.

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Related Institution

Xinjiang Uygur Autonomous Region Hospital of Traditional Chinese Medicine

The Fourth Clinical Medical College of Xinjiang Medical University

The People's Hospital Affiliated to Chongqing Three Gorges Medical College

Institute of Basic Research in Clinical Medicine，China Academy of Chinese Medical Sciences

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