Study on Content of Buddleoside from Flos Chrysanthemi Indici and Their Identification by Infrared

WU Ming-xia 1; WANG Jing-juan2; ZHANG Gui-jun2; SUN Su-qin3

doi:10.13422/j.cnki.syfjx.2010.14.033

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Study on Content of Buddleoside from Flos Chrysanthemi Indici and Their Identification by Infrared

更新时间：2024-09-23

- Study on Content of Buddleoside from Flos Chrysanthemi Indici and Their Identification by Infrared
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 14, Pages: 54-57(2010)
- 作者机构：
  
  1. 河南中医学院
  2. 北京中医药大学
  3. 清华大学化学系
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2010.14.033
  CLC： R284.1
- Published：2010
- 稿件说明：
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[1]吴明侠,王晶娟,张贵君,孙素琴.不同产地野菊花中蒙花苷含量与红外光谱的对比[J].中国实验方剂学杂志,2010,16(14):54-57.

WU Ming-xia 1, WANG Jing-juan2, ZHANG Gui-jun2, et al. Study on Content of Buddleoside from Flos Chrysanthemi Indici and Their Identification by Infrared[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(14): 54-57.
[1]吴明侠,王晶娟,张贵君,孙素琴.不同产地野菊花中蒙花苷含量与红外光谱的对比[J].中国实验方剂学杂志,2010,16(14):54-57. DOI： 10.13422/j.cnki.syfjx.2010.14.033.

WU Ming-xia 1, WANG Jing-juan2, ZHANG Gui-jun2, et al. Study on Content of Buddleoside from Flos Chrysanthemi Indici and Their Identification by Infrared[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(14): 54-57. DOI： 10.13422/j.cnki.syfjx.2010.14.033.

摘要

目的:建立测定野菊花中蒙花苷含量的方法

并且与其红外光谱进行对比研究。方法:以不同产地的供试品为载体

采用HPLC法测定含量

Diamonsil C18色谱柱（4.6mm×250 mm

5μm）;流动相A相为0.5%磷酸水溶液

B相为乙腈

进行梯度洗脱

在0～10 min

B相从10%线性改变至20%

10～30 min

B相从20%线性改变至30%;30～40 min

B相从30%线性改变至40%;流速1.0 mL.min-1;检测波长326 nm;柱温35℃。IR光谱采用傅里叶变换红外光谱技术

获得野菊花的红外谱

二阶导数谱和二维相关红外谱。结果:蒙花苷的线性范围为0.262～5.24μg（r=0.999 91）

回收率为98.75%

RSD为1.02%。不同产地野菊花由于其中所含蒙花苷含量的差异

在IR二阶导数谱上1 700～1 400 cm-1及二维红外光谱在1 800～800 cm-1波段均有明显区别。结论:建立的HPLC方法测定蒙花苷含量准确、快速、重复性好

适合于测定野菊花中黄酮类成分的含量。野菊花中蒙花苷含量的不同在IR光谱上可以看出差异

可以作为鉴别其质量的参考指标之一。

Abstract

Objective:To develop an HPLC method for determination of Buddleoside from Flos Chrysanthemi Indici. The result was compared with IR spectrums. Method:Flos Chrysanthemi Indici from various habitats were used as test sample and HPLC was used. The chromatographic separation was performed on a Diamonsil C18（4. 6 mm × 250 mm

5 μm） column gradient eluted with a mobile phase of 0. 5% H3PO4（A）-acetonitrile（B）

B changed from 10% to 20% in the first 10 min

in the next 20 min

when B changed from 20% to 30%

and in the last 10 min

B changed to 40% . The detection wavelength was set at 326 nm and column temperature was set at 35 ℃ . The FTIR spectrums

the second-order derivative spectrogram and the two-dimensional infrared correlation spectrograms were used. Result:Buddleoside was linear within the range of 0. 262-5. 24 μg （r = 0. 999 91）. The average recovery was 98. 75%

with RSD 1. 02% （n = 5）. There were differences of samples on the second-order derivative spectrogram of Flos Chrysanthemi Indici. in 1 700-1 400 cm -1 and the two-dimensional infrared correlation spectrograms could be classified between frequency range 1 800-800 cm -1. Conclusion:The method was accurate

repetable and suitable to determine buddleoside from Flos Chrysanthemi Indici. The method can be used for quality control of Flos Chrysanthe- mi Indici. IR spectrum can also be used to identify the flavonoids from Flos Chrysanthemi Indici.

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