Determination on Contents of Total Bile Acid in Bile Arisaema by Ultraviolet Gpectrophometry

WAN Jun; ZHAI Yu-jia; YAN Cui-qi; JIA Cheng-cheng

doi:10.13422/j.cnki.syfjx.2010.14.038

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Determination on Contents of Total Bile Acid in Bile Arisaema by Ultraviolet Gpectrophometry

更新时间：2024-09-23

- Determination on Contents of Total Bile Acid in Bile Arisaema by Ultraviolet Gpectrophometry
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 14, Pages: 71-73(2010)
- 作者机构：
  
  西南交通大学生命科学与工程学院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2010.14.038
  CLC： R284.1
- Published：2010
- 稿件说明：
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[1]万军,翟羽佳,闫翠起,贾程程.紫外分光光度法测定胆南星中总胆酸的含量[J].中国实验方剂学杂志,2010,16(14):71-73.

WAN Jun, ZHAI Yu-jia, YAN Cui-qi, et al. Determination on Contents of Total Bile Acid in Bile Arisaema by Ultraviolet Gpectrophometry[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(14): 71-73.
[1]万军,翟羽佳,闫翠起,贾程程.紫外分光光度法测定胆南星中总胆酸的含量[J].中国实验方剂学杂志,2010,16(14):71-73. DOI： 10.13422/j.cnki.syfjx.2010.14.038.

WAN Jun, ZHAI Yu-jia, YAN Cui-qi, et al. Determination on Contents of Total Bile Acid in Bile Arisaema by Ultraviolet Gpectrophometry[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(14): 71-73. DOI： 10.13422/j.cnki.syfjx.2010.14.038.

摘要

目的:建立中药胆南星中总胆酸含量测定的方法。方法:以鹅去氧胆酸为对照品

直接采用加入50%硫酸溶液

75℃水浴加热反应显色

于紫外分光光度计（377±2）nm波长处测定。结果鹅去氧胆酸在0.000 0～0.431 2 g.L-1呈良好线性关系（r=0.999 1）

平均回收率为98.79%（n=5）

RSD为3.02%。结论本方法准确

简便

重复性好

可用于中药胆南星中总胆酸含量测定。

Abstract

Objective:To establish the methods of determination on the contents of total bile acid in Bile Arisaema by ultraviolet spectrophometry.Method:To add 50% sulfuric acid to the sample and react in water bath at 75 ℃.The contents of total bile acid were determined by ultraviolet spectrophometry on（377 ± 2） nm

using chenodeoxycholic acid（CDCA） as the chemical reference substance.Result:The linear response range was 0.000 0 ～ 0.431 2 g.L-1

r = 0.999 1.The average recovery was 98.79%（n = 5）

RSD 3.02%.Conclusion:The method was accurate

simple and reproducible which could be used for the determination of total bile acid in Bile Arisaema.

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Related Author

WAN Jun

ZHAI Yu-jia

YAN Cui-qi

JIA Cheng-cheng

SHAO Juan

XU Mengdan

ZHANG Xuejing

GUO Yunlong

Related Institution

School of Pharmaceutical Sciences， Changchun University of Chinese Medicine

Jilin Ginseng Academy，Changchun University of Chinese Medicine

Sunflower Pharmaceutical Group（Xiangyang） Longzhong Co. Ltd.，Xiangyang

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences，Beijng

Shanxi University of Chinese Medicine

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