Effect of Dihuang Yinzi on Hypertension Development in Spontaneously Hypertensive Rats:Role of Improving Endothelial Function

WANG Xian-zhen1; JIANG Jia-ye1; LUO Shan-shan1; WANG Xun1; LU Jia-feng1; BIAN Ka2; KE Yan1

doi:10.13422/j.cnki.syfjx.2010.14.051

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Effect of Dihuang Yinzi on Hypertension Development in Spontaneously Hypertensive Rats:Role of Improving Endothelial Function

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- Effect of Dihuang Yinzi on Hypertension Development in Spontaneously Hypertensive Rats:Role of Improving Endothelial Function
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 14, Pages: 110-114(2010)
- 作者机构：
  
  1. 上海中医药大学教学实验中心
  2. 上海高校一氧化氮与炎症医学研究院
  3. 美国德克萨斯大学休斯顿医学院综合生物及药理学系,德克萨斯大学分子医学研究所
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2010.14.051
  CLC： R285.5
- Published：2010
- 稿件说明：
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[1]王现珍,蒋嘉烨,罗珊珊,王勋,陆家凤,卞卡,可燕.地黄饮子改善自发性高血压大鼠进程中血管内皮功能的研究[J].中国实验方剂学杂志,2010,16(14):110-114.

WANG Xian-zhen1, JIANG Jia-ye1, LUO Shan-shan1, et al. Effect of Dihuang Yinzi on Hypertension Development in Spontaneously Hypertensive Rats:Role of Improving Endothelial Function[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(14): 110-114.
[1]王现珍,蒋嘉烨,罗珊珊,王勋,陆家凤,卞卡,可燕.地黄饮子改善自发性高血压大鼠进程中血管内皮功能的研究[J].中国实验方剂学杂志,2010,16(14):110-114. DOI： 10.13422/j.cnki.syfjx.2010.14.051.

WANG Xian-zhen1, JIANG Jia-ye1, LUO Shan-shan1, et al. Effect of Dihuang Yinzi on Hypertension Development in Spontaneously Hypertensive Rats:Role of Improving Endothelial Function[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(14): 110-114. DOI： 10.13422/j.cnki.syfjx.2010.14.051.

摘要

目的:探讨自发性高血压大鼠（SHR）高血压发展进程中

地黄饮子对其血管内皮功能的修复情况及作用机制研究。方法:54只雄性SHR随机分为3组:模型组、地黄饮子组和卡托普利组

7～24周龄ig地黄饮子（生药15.9 g.kg-1.d-1）和卡托普利（3.375 g.kg-1.d-1）

停药观察至32周龄。18

32周龄时分别颈动脉插管测定大鼠血压值

血管等长张力实验检测离体肠系膜上动脉乙酰胆碱（Ach）依赖性舒张功能;Griess法检测血清NO 2-水平;实时荧光定量PCR（Real-time PCR）检测动脉组织白介素-1（IL-1）

白介素-6（IL-6）

肿瘤坏死因子-α（TNF-α）和iNOS mRNA)的表达。结果:地黄饮子降低SHR 18周龄血压

增加18

24周龄肠系膜上动脉Ach依赖性舒张功能和血清NO 2-含量

显著降低动脉组织IL-1

IL-6

TNF-α和iNOSmRNA表达;卡托普利降压作用优于地黄饮子

停药后作用消失

无改善肠系膜上动脉Ach依赖性舒张功能作用

增加血清24周龄NO 2-水平和动脉组织IL-1

IL-6 mRNA表达的同时降低TNF-α和iNOS表达。结论:地黄饮子能显著改善SHR肠系膜上动脉内皮功能

表现为较好的抗高血压作用。该作用可能与其抑制IL-1

IL-6

TNF-α和iNOS表达

改善血管微环境的炎症反应状态有关。

Abstract

Objective:To investigate the action mechanism of Dihuang Yinzi on hypertension progress of spontaneously hypertensive rats（SHR）

and understand the role of endothelial function recovery by Dihuang Yinzi.Method:Fifty-four male SHR were divided into three groups:the control;Dihuang Yinzi treatment;and captoril treatment.Dihuang Yinzi（crude drug 15.9 g.kg-1.d-1） and captoril（3.375 g.kg-1.d-1） were treated from wk 7 to wk 24（wk = week） through gavage.The drug effect was observed at wk 18 and wk 24 during the treatments and 8 wk（wk32） after treatment.Experiments including the blood pressure by arterial carotis cannula;the concentration of NO 2-by Griess;the Ach-dependent relaxation of mesenteric rings by isometric tension change;and the mRNA expressions of IL-1

IL-6

TNF-α and iNOS of aorta tissue by real time PCR.Result:Dihuang Yinzisignificantly reduced blood pressure at wk18

and increased Ach-dependent relaxation of mesenteric rings and the NO 2-concentration in serum at wk18 and wk24.The mRNA expression of IL-1

IL-6

TNF-α and iNOS was markedly inhibited by the herb formula.Although captopril showed stronger anti-hypertensive effect

which could not extended after drug withdraw.In addition

the beta blocker did not enhance Ach-dependent vasodilation.NO 2-levels in serum and IL-1

IL-6 expression of aorta tissue were increased by captopril

while iNOS and TNF-α were inhibited at wk 24.Conclusion:Dihuang Yinzi showed anti-hypertensive effect with enhanced endothelium-dependent vasodilation in mesenteric artery and increased NO 2-concentration in serum.Furthermore

the herb formula also exerted anti-inflammatory effect by decreasing IL-1

IL-6

TNF-α and iNOS expression.

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Related Author

WANG Xian-zhen

JIANG Jia-ye

LUO Shan-shan

WANG Xun

LU Jia-feng

BIAN Ka

KE Yan

YANG Pi-jian

Related Institution

The First Clinical Medical College of Shaanxi University of Chinese Medicine

Experimental Research Center，China Academy of Chinese Medical Sciences

Beijing University of Chinese Medicine

Guangdong Metabolic Diseases Research Center of Integrated Chinese and Western Medicine， Guangdong Pharmaceutical University

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

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