Comparison of Experimental Renal Calculus of Calcium Oxalate in Rats

LAI Hai-biao1; LIU Zhao-hui1; WU Song1; OU Xiu-hua2; GU Xiang-ming1; WANG Shu-qin1; GAO Yu-qiao1; LIN Hui1; WANG Yun1; DAI Wei-bo1; FAN Wen-chang1; QIU Lan-ying1; LIN Jia-le1; MEI Quan-xi1

doi:10.13422/j.cnki.syfjx.2010.14.057

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Comparison of Experimental Renal Calculus of Calcium Oxalate in Rats

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- Comparison of Experimental Renal Calculus of Calcium Oxalate in Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 14, Pages: 135-138(2010)
- 作者机构：
  
  1. 中山市中医院
  2. 广州中医药大学
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2010.14.057
  CLC： R285.5
- Published：2010
- 稿件说明：
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[1]赖海标,刘朝晖,吴松,欧秀华,顾向明,王书芹,高玉桥,林慧,汪芸,戴卫波,范文昌,丘兰英,林家乐,梅全喜.实验性大鼠肾草酸钙结石造模方法筛选研究[J].中国实验方剂学杂志,2010,16(14):135-138.

LAI Hai-biao1, LIU Zhao-hui1, WU Song1, et al. Comparison of Experimental Renal Calculus of Calcium Oxalate in Rats[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(14): 135-138.
[1]赖海标,刘朝晖,吴松,欧秀华,顾向明,王书芹,高玉桥,林慧,汪芸,戴卫波,范文昌,丘兰英,林家乐,梅全喜.实验性大鼠肾草酸钙结石造模方法筛选研究[J].中国实验方剂学杂志,2010,16(14):135-138. DOI： 10.13422/j.cnki.syfjx.2010.14.057.

LAI Hai-biao1, LIU Zhao-hui1, WU Song1, et al. Comparison of Experimental Renal Calculus of Calcium Oxalate in Rats[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(14): 135-138. DOI： 10.13422/j.cnki.syfjx.2010.14.057.

摘要

目的:筛选一种方法简便、稳定、成石效果好的实验性大鼠肾草酸钙结石造模方法。方法:采用几种目前常用的实验性大鼠肾草酸钙结石造模方法（造模Ⅰ组:1%乙二醇饮水+2%氯化铵每天ig 2 mL/只;造模Ⅱ组:1.25%乙二醇+1%氯化铵

ig 20 mL.kg-1.d-1;造模Ⅲ组:每只每天饮用1.25%乙二醇+1%氯化铵10 mL

水30 mL;造模Ⅳ组:1%乙二醇+1%氯化铵自由饮用）进行造模

并进行各组尿液、血液和肾组织中的多项生化指标【尿素氮（BUN）、肌酐（Cr）、尿酸（UA）、无机磷（IP）、钙（Ca2+）、草酸（OX）】检测以及肾脏病理标本观察

综合评价造模效果。结果:造模Ⅰ组的24 h尿液中的BUN

Ca2+

OX均高于正常对照组（Ⅴ组）

且统计学上有极显著性差异（P<0.01）;血液中BUN

Ca2+均高于正常对照组

且统计学上有极显著性差异（P<0.01）;肾组织中Ca2+和OX均高于正常对照组

且统计学上有极显著性差异（P<0.01）;肾组织可见大量草酸钙结晶沉积。造模Ⅱ组和造模Ⅲ组的多项生化指标结果显示大鼠的肾功能下降

但肾组织中未见结晶体

仅发现肾间质血管充血。造模Ⅳ组大鼠全部死亡。结论:造模Ⅰ组为最佳造模方法。

Abstract

Objective:To select a simple

stable and satisfied experimental model of renal calcium oxalate calculus in rats.Method:After several renal calcium oxalate calculus models currently used（Model group I:administered intragastrically with 1% ethylene glycol free drink + 2% ammonium chloride 2 mL/rat per day;model Group Ⅱ:administered intragastrically with 1.25% ethylene glycol + 1% ammonium chloride 20 mL.kg-1 per day;model group Ⅲ:drank 10 mL 1.25% ethylene glycol + 1% ammonium chloride and 30 mL water per day;model group Ⅳ:drank 1% ethylene glycol + 1% ammonium chloride freely.） experimental rat models were developed.Several biochemical indicators[urea nitrogen（BUN）

creatinine（Cr）

uric acid（UA）

inorganic phosphorus（IP）

calcium（Ca2 +）

oxalate（OX）] of urine

blood and nephridial tissue were measured and renal pathological specimens were observed.Then the modeling results were comprehensive evaluated.Result:The 24 h urinary BUN

Ca2 +

OX of model groupⅠwere singnificantly higher than the normal control group（groupⅤ）（P < 0.01）;blood BUN

Ca2 + were significantly higher than the normal control group（P < 0.01）;nephridial tissue Ca2 + and OX were significantly higher than the normal control group（P < 0.01）;and calcium oxalate crystals were distributed in nephridial tissue.The several biochemical indicators of model group Ⅱ and Model group Ⅲ disclosed the rats’ renal function breakdown

and renal pathological specimens only displayed vascular engorgement in renal interstitium and calcium oxalate crystals hadn’t been seen in nephridial tissue.All rats of model group Ⅳ were dead.Conclusion:Model groupⅠrepresents the best modeling for renal calculus of calcium oxalate.

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