Influence of Ease Powder Decoction on Expression of Hippocampus 5-HT<sub>1A</sub> Receptor and 5-HT<sub>2A</sub> Receptor in Rat Model of Sleep Deprivation Depression

KONG Mei; XING Chang-yong; SHU Xiao-chun

doi:10.13422/j.cnki.syfjx.2010.14.063

您当前的位置：

首页 >

文章列表页 >

Influence of Ease Powder Decoction on Expression of Hippocampus 5-HT_1A Receptor and 5-HT_2A Receptor in Rat Model of Sleep Deprivation Depression

更新时间：2024-09-23

- Influence of Ease Powder Decoction on Expression of Hippocampus 5-HT_1A Receptor and 5-HT_2A Receptor in Rat Model of Sleep Deprivation Depression
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 14, Pages: 157-160(2010)
- 作者机构：
  
  中山大学第五附属医院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2010.14.063
  CLC： R285.5
- Published：2010
- 稿件说明：
移动端阅览
[1]孔梅,邢长永,舒晓春.逍遥散干预抑郁症睡眠障碍模型大鼠海马5-HT_(1A)受体,5-HT_(2A)受体的变化[J].中国实验方剂学杂志,2010,16(14):157-160.

KONG Mei, XING Chang-yong, SHU Xiao-chun. Influence of Ease Powder Decoction on Expression of Hippocampus 5-HT_1A Receptor and 5-HT_2A Receptor in Rat Model of Sleep Deprivation Depression[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(14): 157-160.
[1]孔梅,邢长永,舒晓春.逍遥散干预抑郁症睡眠障碍模型大鼠海马5-HT_(1A)受体,5-HT_(2A)受体的变化[J].中国实验方剂学杂志,2010,16(14):157-160. DOI： 10.13422/j.cnki.syfjx.2010.14.063.

KONG Mei, XING Chang-yong, SHU Xiao-chun. Influence of Ease Powder Decoction on Expression of Hippocampus 5-HT_1A Receptor and 5-HT_2A Receptor in Rat Model of Sleep Deprivation Depression[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(14): 157-160. DOI： 10.13422/j.cnki.syfjx.2010.14.063.

摘要

目的:观察逍遥散干预抑郁症睡眠障碍（SDD）大鼠自主行为及海马5羟色胺1A受体（5-HT1A受体）

5羟色胺2A受体（5-HT2A受体）mRNA表达变化

探讨其治疗SDD的分子生物学机制。方法:48只Wistar大鼠进行敞箱行为（OFT）试验

根据得分将大鼠随机分为4组:正常组、模型组、米氮平组、逍遥散组。除正常组外

其余各组共接受18 d不同的应激

应激18 d后

进行72 h快眼睡眠剥夺造模（DEM）。每天在应激前1 h按组给药;模型组、米氮平组、逍遥散组分别予等容量生理盐水、米氮平、逍遥散水提液。21 d计算行为得分后处死大鼠

取大鼠海马置液氮中备测。结果:21 d后模型组大鼠自主活动较应激前显著减少（P<0.01）。与模型组比较

逍遥散组及米氮平组大鼠的自主活动明显增加

已达正常组水平;与正常组比较

模型组海马5-HT1AR mRNA表达水平下降

5-HT2AR mRNA表达水平上升（P<0.01）;逍遥散组海马5-HT1AR mRNA

5-HT2ARmRNA的表达改善明显

与模型组比较

（P<0.01）;而米氮平组与模型组比较无统计学意义

逍遥散组作用明显优于米氮平组（P<0.05）。结论:逍遥散可能通过上调海马5-HT1AR

下调5-HT2AR

调节5-HT再摄取发挥治疗SDD效应。

Abstract

Objective:To discuss the molecular mechanism of Ease Powder decoction treating on sleep deprivation depression（SDD） by observing the changes of autonomic-behavior and the expression of hippocampus 5-HT1A receptor and 5-HT2A receptor in a rat experimental model of sleep deprivation depression.Method:48 Wistar rats were divided into four groups after first open-field testing（OFT）

they were normal group

model group

mirtazapine group and Ease Powder decoction group.Except the normal group

the other groups were received different stress for 21 days

then made 72 h rapid eye movement（REM）

taken the drug every day before the stress（1 h）.Meanwhile

the model group

mirtazapine group and Ease Powder decoction group were intragastric administration of normal saline

mirtazapine

Ease Powder decoction respectively.At the end of 21 days

all the animals were sacrificed.The hippocampus of rats were taken then kept in liquid nitrogen.Result:Compared with the situation before stress

there is a significant decrease in the autonomic-behavior of SDD after 21 days（P <0.01）.Compared with the model group

the rat’s autonomic-behavior in Ease Powder decoction group and the mirtazapine group is increased.Compared with the normal group

the expression of 5-HT1AR mRNA in hippocampus of model group is decreased

the expression of 5-HT2A R mRNA is increased

with statistical significance（P < 0.05）.Comparison for expression of hippocampus 5-HT1AR mRNA and 5-HT2AR mRNA between the Ease Powder decoction group and the model group is significant（P < 0.01）.However

compared with the model group

the mirtazapine group has no statistical significance.Comparison of Ease Powder decoction group and mirtazapine group has statistical significance（P < 0.05）.Conclusion:The Ease Powder decoction exert an effect on SDD by regulating the expression of hippocampus 5-HT1AR and 5-HT2AR.

关键词

Keywords

references

Views

666

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Influence of Ease Powder Decoction on Expression of Hippocampus 5-HT Receptor and 5-HT Receptor in Rat Model of Sleep Deprivation Depression

Expression of Serum Thyroxine in a Rat Model of Sleep Deprivation Depression and Influenced of Ease Powder Decoction

Biological Basis of Premature Ejaculation Based on Theory of "Brain-heart-kidney-semen Chamber" Axis

Traditional Chinese Medicine in Treatment of Constipation Based on 5-HT Signaling Pathway： A Review

Syndrome Differentiation-based Treatment of Diarrhea-predominant Irritable Bowel Syndrome with Chinese Medicine via 5-HT Signaling Pathway： A Review

Related Author

KONG Mei

XING Chang-yong

SHU Xiao-chun

MA Dongyue

WANG Anmin

YANG Jiutian

LUO Dicheng

Related Institution

Xiyuan Hospital， China Academy of Chinese Medical Sciences

Third Affiliated Hospital of Liaoning University of TCM

Liaoning University of Traditional Chinese Medicine（TCM）

The Affiliated Hospital of Shaanxi University of Chinese Medicine

Shaanxi University of Chinese Medicine

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰

Influence of Ease Powder Decoction on Expression of Hippocampus 5-HT1A Receptor and 5-HT2A Receptor in Rat Model of Sleep Deprivation Depression

DOI：10.13422/j.cnki.syfjx.2010.14.063

摘要

Abstract

关键词

Keywords

references

Influence of Ease Powder Decoction on Expression of Hippocampus 5-HT_1A Receptor and 5-HT_2A Receptor in Rat Model of Sleep Deprivation Depression