Metabonomic Analysis of Urine from Bronchial Asthma in Cavia Cobaya Treated by Acupoint External Application with ‘Treating Winter Disease in Summer’Prescription

LIU Li1; LI Hui1; SUN Xue-gang1; CHEN Zhi-liang2; LIU Qiang1﹡

doi:10.13422/j.cnki.syfjx.2010.15.002

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Metabonomic Analysis of Urine from Bronchial Asthma in Cavia Cobaya Treated by Acupoint External Application with ‘Treating Winter Disease in Summer’Prescription

更新时间：2024-09-23

- Metabonomic Analysis of Urine from Bronchial Asthma in Cavia Cobaya Treated by Acupoint External Application with ‘Treating Winter Disease in Summer’Prescription
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 15, Pages: 180-183(2010)
- 作者机构：
  
  1. 南方医科大学中医药学院
  2. 南方医科大学附属南方医院药学部
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2010.15.002
  CLC： R244.9
- Published：2010
- 稿件说明：
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[1]刘莉,李慧,孙学刚,陈志良,刘强.“冬病夏治”方药穴位贴敷治疗哮喘豚鼠的尿液代谢组学研究[J].中国实验方剂学杂志,2010,16(15):180-183.

LIU Li1, LI Hui1, SUN Xue-gang1, et al. Metabonomic Analysis of Urine from Bronchial Asthma in Cavia Cobaya Treated by Acupoint External Application with ‘Treating Winter Disease in Summer’Prescription[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(15): 180-183.
[1]刘莉,李慧,孙学刚,陈志良,刘强.“冬病夏治”方药穴位贴敷治疗哮喘豚鼠的尿液代谢组学研究[J].中国实验方剂学杂志,2010,16(15):180-183. DOI： 10.13422/j.cnki.syfjx.2010.15.002.

LIU Li1, LI Hui1, SUN Xue-gang1, et al. Metabonomic Analysis of Urine from Bronchial Asthma in Cavia Cobaya Treated by Acupoint External Application with ‘Treating Winter Disease in Summer’Prescription[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(15): 180-183. DOI： 10.13422/j.cnki.syfjx.2010.15.002.

摘要

目的:采用代谢组学方法

研究"冬病夏治"方药穴位贴敷法治疗哮喘豚鼠的机制。方法:通过超高效液相色谱结合高分辨率质谱联用（UPLC-Q-TOF-MS）技术分析空白组、卵蛋白致敏的豚鼠哮喘模型组及中药穴位贴敷组豚鼠尿液代谢物图谱.使用SIMCA-P+软件正交偏最小二乘判别分析法（PLS-DA）对采集的数据进行处理。结果:哮喘模型组和空白组、穴位给药组与哮喘模型组分别呈现聚类分布

2组之间未见任何交叉与重叠

得到了明显区分。初步确认氮4-乙酰基胞苷（N4-acetylcytidine

ac4c）、6-甲基腺苷（6-methyladinosinek

m6a）以及未确定化合物（荷质比为365.14）为哮喘发作潜在的生物标志物。甲基戊二酸、1-甲基次黄苷为冬病夏治方治疗哮喘潜在的生物标志物。结论:本研究建立了基于豚鼠尿液的UPLC-Q-TOF-MS代谢组学研究方法

初步阐释了"冬病夏治"方药治疗哮喘的代谢组学机制。研究结果对用现代生物科学技术解释传统中药制剂理论有一定的启发作用。

Abstract

Objective: To study the mechanism of acupoint external application with "treating winter disease in summer"prescription on bronchial asthma in cavia cobaya.Method: The metabolite profiles of the urine samples of the blank control group

the asthma-model group and the acupoint administration group were detected by ultraperformance liquid chromatography coupled with Q-TOF mass spectrometry（UPLC-Q-TOF-MS）.The data was analyzed by partial least square discriminatory analysis（PLS-DA） in SIMCA-P +.Result: The result showed that metabolite profile of the urine sample was separated obviously and had no any overlapping between the asthma-model group and the blank control group

the acupoint administration group and the asthma-model group.N4-acetylcytidine

6-methyladinosinek and an undetermined compound were confirmed initially as the marker of asthma

while methylglutarate and methyl inosine

the markers of the ‘treating winter disease in summer’prescription.Conclusion: The topic establishes a method of metabonomic analysis for urine from cavia cobaya using UPLC-Q-TOF-MS.The study shows the mechanism of metabolism for the therapy of asthma with‘treating winter disease in summe’prescription.The results will be of great helpful to explain the theory of Chinese drugs pharmaceutics using biological science of modern time.

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Related Author

LIU Li

LI Hui

SUN Xue-gang

CHEN Zhi-liang

LIU Qiang

YANG Shuo

LI Haiyan

XIE Yanming

Related Institution

Institute of Information on Traditional Chinese Medicine，China Academy of Chinese Medical Sciences

Institute of Basic Research in Clinical Medicine，China Academy of Chinese Medical Sciences

Henan Provincial Hospital of Traditional Chinese Medicine（TCM）/Second Affiliated Hospital of Henan University of TCM

Putuo Hospital， Shanghai University of TCM

Children's Hospital of Shanghai， Children's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine

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