Separation and Purification of Total Flavonoids of BYHWD by Macroporous Resin

XING Pan-ke; YANG Jie-hong; ZHANG Yu-yan; WAN Hai-tong; LI Peng

doi:10.13422/j.cnki.syfjx.2010.15.005

您当前的位置：

首页 >

文章列表页 >

Separation and Purification of Total Flavonoids of BYHWD by Macroporous Resin

更新时间：2024-09-23

- Separation and Purification of Total Flavonoids of BYHWD by Macroporous Resin
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 15, Pages: 1-4(2010)
- 作者机构：
  
  浙江中医药大学
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2010.15.005
  CLC： R284.1
- Published：2010
- 稿件说明：
移动端阅览
[1]邢攀科,杨洁红,张宇燕,万海同,李鹏.大孔吸附树脂分离纯化补阳还五汤中总黄酮[J].中国实验方剂学杂志,2010,16(15):1-4.

XING Pan-ke, YANG Jie-hong, ZHANG Yu-yan, et al. Separation and Purification of Total Flavonoids of BYHWD by Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(15): 1-4.
[1]邢攀科,杨洁红,张宇燕,万海同,李鹏.大孔吸附树脂分离纯化补阳还五汤中总黄酮[J].中国实验方剂学杂志,2010,16(15):1-4. DOI： 10.13422/j.cnki.syfjx.2010.15.005.

XING Pan-ke, YANG Jie-hong, ZHANG Yu-yan, et al. Separation and Purification of Total Flavonoids of BYHWD by Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(15): 1-4. DOI： 10.13422/j.cnki.syfjx.2010.15.005.

摘要

目的:探究大孔吸附树脂法分离纯化补阳还五汤中总黄酮的最佳工艺条件和参数。方法:通过静态吸附及解吸试验、动态吸附及洗脱试验

从D101

DA201

AB-8和聚酰胺树脂中筛选出适合补阳还五汤总黄酮分离的理想树脂。并通过单因素分析考察该树脂分离、纯化补阳还五汤总黄酮的最佳工艺条件。结果:经D101大孔吸附树脂纯化后的补阳还五汤总黄酮纯度最高

达到63.1%

转移率为80.2%

静态吸附容量为66.28 mg·g-1（干树脂）

最佳动态上样量为20 mL·g-1（总黄酮质量浓度:3.033 g·L-1）

上样流速为1 BV·h-1

洗脱乙醇体积分数为60%

洗脱速度为1.5 BV·h-1。结论:D101大孔吸附树脂可较好地用于补阳还五汤总黄酮的分离纯化。

Abstract

Objective:To investigate the optimum conditions and parameters of macro reticular resin in purification of flavonoids in BYHWD.Method:Through the static adsorption and desorption experiments and the dynamic adsorption and desorption experiments

the ideal resins for BYHWD flavonoids from the D101

DA201

AB-8 and polyamide were screened.Through single-factor analysis to study the optimum conditions of resin in separating and purifying BWD flavonoids.Result:After purification by D101

BYHWD the purity of flavonoids could reach 63.1%

transfer rate was 80.2%.The static adsorption capacity of D101 macroporous resin for BYHWD flavonoids was 66.28 mg.g-1（dry resin）

the best dynamics on the sample size was 20 mL.g-1（total flavonoids concentration 3.033 mg.mL-1）

the sample flow rate was 1 BV.h-1

elution ethanol concentration was 60%

elution speed was 1.5 BV.h-1.Conclusion: D101 macroreticular resin could be better used for the separation and purification of BWD flavonoids.

关键词

Keywords

references

Views

199

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Studies on Purification of Saponins (PNS) with Macroporous Resin

Study on Separation and Purification Total Glycosides of Tree Peony Bark by Macroporous Resin

Purification of Total Alkaloid from Rhizoma Coptidis by Weakly Acidic Ion Exchange Resin

Related Author

XING Pan-ke

YANG Jie-hong

ZHANG Yu-yan

WAN Hai-tong

LI Peng

LIU Zhong-qiu

CAI Xiong

LAI Xiao-ping

Related Institution

School of Pharmacy,Shandong University of Traditional Chinese Medicine,Ji’nan

Heilongjiang University of Chinese Medicine

School of Pharmacy，Tianjin University of Traditional Chinese Medicine

School of Pharmacy， Jiangxi University of Chinese Medicine

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰