YANG Jun-xuan1, YIN Rong-li1, CHEN Jin-yu1, et al. Study on Purification of Chlorogenic Acid from Lonicera Japonica Leaves with Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(15): 20-23.
YANG Jun-xuan1, YIN Rong-li1, CHEN Jin-yu1, et al. Study on Purification of Chlorogenic Acid from Lonicera Japonica Leaves with Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(15): 20-23. DOI: 10.13422/j.cnki.syfjx.2010.15.008.
Study on Purification of Chlorogenic Acid from Lonicera Japonica Leaves with Macroporous Resin
药液质量浓度0.5~1.0 g·mL-1、吸附速度0.5 BV·h-1、树脂吸附量生药0.3 g·g-1、洗脱溶媒30%乙醇、洗脱速度2 B V·h-1
洗脱溶媒用量2 BV。结论:HPD450大孔树脂对绿原酸纯化效果较好
工艺稳定可行
可用于工业化生产。
Abstract
Objective:To establish a method for determination of chlorogenic acid in Lonicera japonica leaves by HPLC
and study on extracting and purifying chlorogenic acid from Lonicera japonica leaves by different macroporous resin
so as to provide a reference for the comprehensive development and utilization of honeysuckle herbs.Method: The chlorogenic acid was determined with the detection wavelength 327 nm by HPLC.Static adsorption and dynamic adsorption were used to optimize the adsorption and elution process by macroporous resin.Result:Chlorogenic acid was linear in the range of 2.376 8-60.476 9 μg.The average recovery was 96.82%
RSD was 1.83%.At the same time
the purification effect of chlorogenic acid by HPD450 macroporous resin was better than other resins
the optimal conditions were:column diameter ratio 3 ∶ 1
liquid concentration 0.5-1.0 g·mL-1
absorption velocity 0.5 BV·h-1
resin adsorption quantity 0.3 g·g-1
elution solvent 30% ethanol
elution speed 2 BV·h-1
elution solvent volume 2 BV.Conclusion:The process of purification of chlorogenic acid from Lonicera japonica leaves by HPD450 macroporous resin was stable and could be used for industrial production.