Simultaneous Determination of Five Hydrophilic and Lipophilic Active Components in Radix et Rhizoma Salviae Miltiorrhizae by HPLC

ZHANG Hui-xian1; DU Shou-ying1; LU Yang1; CHEN Xiao-lan1; SONG Xiao1; LI Dong-xue2; XU Bing1

doi:10.13422/j.cnki.syfjx.2010.15.020

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Simultaneous Determination of Five Hydrophilic and Lipophilic Active Components in Radix et Rhizoma Salviae Miltiorrhizae by HPLC

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- Simultaneous Determination of Five Hydrophilic and Lipophilic Active Components in Radix et Rhizoma Salviae Miltiorrhizae by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 15, Pages: 34-38(2010)
- 作者机构：
  
  1. 北京中医药大学中药学院
  2. 中国生物技术发展中心
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2010.15.020
  CLC： R284.1
- Published：2010
- 稿件说明：
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[1]张慧娴,杜守颖,陆洋,陈晓兰,宋逍,李冬雪,徐冰.HPLC同时测定丹参水溶性及脂溶性5种成分的含量[J].中国实验方剂学杂志,2010,16(15):34-38.

ZHANG Hui-xian1, DU Shou-ying1, LU Yang1, et al. Simultaneous Determination of Five Hydrophilic and Lipophilic Active Components in Radix et Rhizoma Salviae Miltiorrhizae by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(15): 34-38.
[1]张慧娴,杜守颖,陆洋,陈晓兰,宋逍,李冬雪,徐冰.HPLC同时测定丹参水溶性及脂溶性5种成分的含量[J].中国实验方剂学杂志,2010,16(15):34-38. DOI： 10.13422/j.cnki.syfjx.2010.15.020.

ZHANG Hui-xian1, DU Shou-ying1, LU Yang1, et al. Simultaneous Determination of Five Hydrophilic and Lipophilic Active Components in Radix et Rhizoma Salviae Miltiorrhizae by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(15): 34-38. DOI： 10.13422/j.cnki.syfjx.2010.15.020.

摘要

目的:建立同时测定丹参中2种水溶性和3种脂溶性成分的HPLC方法。方法:采用C18柱（4.6 mm×250 mm

5μm）

以乙腈（A）-0.05%磷酸水溶液（B）为流动相进行梯度洗脱:0～27 min

20%～25%A;27～55 min

25%～80%A;55～65min

80%～80%A;检测波长为270 nm和286 nm

柱温30℃。结果:迷迭香酸、丹酚酸B、隐丹参酮、丹参酮Ⅰ、丹参酮ⅡA分别在1.92×10-2～0.96μg

7.29×10-2～3.64μg

3.05×10-3～0.15μg

1.71×10-3～8.55×10-2μg

5.80×10-3～0.29μg呈良好的线性关系。加样回收率分别为99.88%

104.75%

101.08%

101.41%

102.41%。结论:该含量测定方法准确、稳定、简便

分离效果好

能同时测定丹参中迷迭香酸、丹酚酸B、隐丹参酮、丹参酮Ⅰ、丹参酮ⅡA 5种有效成分的含量。

Abstract

Objective: To develop a new method for simultaneous determination of two hydrophillc components and three lipophillc components of Radix et Rhizoma Salviae Miltiorrhizae.Method:The HPLC-double wavelength detection was employed using a column of Dikma Diamonsil C18（4.6 mm × 250 mm

5 μm） with a mobile phase of acetonitrile（A）-0.05% phosphoric acid（B）.The gradient elution program was as follows:0-27 min

20%-25% A;27-55 min

25%-80% A;55-65 min

80%-80% A.The detection wavelength was set at 270 nm and 286 nm.The column temperature was at 30 ℃.Result:Rosmarinic acid was linear in the range of 1.92 × 10-2-0.96 μg;salviamolic acid B was linear in the range of 7.29 × 10-2-3.64 μg;clyptotanshinon was linear in the range of 3.05 × 10-3-0.15 μg;tanshinone was linear in the range of 1.71 × 10-3-8.55 × 10-2 μg and tanshinone ⅡA was linear in the range of 5.80 × 10-3-0.29 μg.The average recovery was 99.88%

104.75%

101.08%

101.41%

102.41% respectively.Conclusion:The present method was simple and had satisfactory efficacy;it can simultaneously determine multiple hydrophilic and lipophilic bioactive components in Salvia miltiorrhiza from different areas.

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Related Author

ZHANG Hui-xian

DU Shou-ying

LU Yang

CHEN Xiao-lan

SONG Xiao

LI Dong-xue

XU Bing

WANG Li-fang

Related Institution

Institute of Traditional Chinese Medicine， Heilongjiang University of Chinese Medicine

Graduate School，Heilongjiang University of Chinese Medicine

Shandong University of Traditional Chinese Medicine

Institue of Chinese Materia Medica, China Academy of Chinese Medical Sciences

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