Determination of Magnolia and Honokiol in Magnolia officinalis Processed by Different Methods and from Different Areas by HPLC

DING Guang-yu; ZHANG Zhen-qiu

doi:10.13422/j.cnki.syfjx.2010.15.030

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Determination of Magnolia and Honokiol in Magnolia officinalis Processed by Different Methods and from Different Areas by HPLC

更新时间：2024-09-23

- Determination of Magnolia and Honokiol in Magnolia officinalis Processed by Different Methods and from Different Areas by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 15, Pages: 66-68(2010)
- 作者机构：
  
  辽宁中医药大学药学院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2010.15.030
  CLC： R283
- Published：2010
- 稿件说明：
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[1]丁广宇,张振秋.不同炮制方法对厚朴中厚朴酚与和厚朴酚含量的影响[J].中国实验方剂学杂志,2010,16(15):66-68.

DING Guang-yu, ZHANG Zhen-qiu. Determination of Magnolia and Honokiol in Magnolia officinalis Processed by Different Methods and from Different Areas by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(15): 66-68.
[1]丁广宇,张振秋.不同炮制方法对厚朴中厚朴酚与和厚朴酚含量的影响[J].中国实验方剂学杂志,2010,16(15):66-68. DOI： 10.13422/j.cnki.syfjx.2010.15.030.

DING Guang-yu, ZHANG Zhen-qiu. Determination of Magnolia and Honokiol in Magnolia officinalis Processed by Different Methods and from Different Areas by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(15): 66-68. DOI： 10.13422/j.cnki.syfjx.2010.15.030.

摘要

目的:测定不同炮制品及地区厚朴中有效成分（厚朴酚与和厚朴酚）含量

进行考察

为制定厚朴的质量标准提供依据。方法:采用HPLC法对不同炮制品及地区厚朴中厚朴酚与和厚朴酚的含量进行测定。色谱柱Phenomsil C18柱（4.6mm×250 mm

5μm）

流动相甲醇-水（78∶22）

流速1 mL·min-1

柱温25℃

检测波长294 nm.进样量10μL。结果:不同炮制品及地区的厚朴在有效成分含量上有一定差异。结论:该方法简便、准确、分离效果好

可以用来测定厚朴中有效成分的含量。

Abstract

Objective:To determine the contents of active ingredients（magnolia and honokiol） in Magnolia officinalis processed by different methods and from different areas by HPLC.Method: HPLC was applied to determine the contents of magnolia and honokiol in M.officinalis processed by different methods and from different areas.The samples were performed on a Phenomsil C18（4.6 mm × 250 mm

5 μm） column with mobile phase of CH3OH-H2O（78∶22）at a flow rate of 1.0 mL·min-1.The detection wavelength was set at 294 nm.The column temperature was 25 ℃ and the injection volumn was 10 μL.Result:There are some difference on contents of active ingredients in M.officinalis processed by different methods and from different areas.Conclusion:The method is convenient.accurate and suitale for separation of magnolia and honokiol.It can be used for determination of magnolia and honokiol in M.officinalis.

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Related Author

DING Guang-yu

ZHANG Zhen-qiu

LIU Ying-xin

LIU Li-li

YU Zu-wen

LI Fei-yan

XIAO Lukun

ZHAO Anyi

Related Institution

School of Pharmacy，Shanxi Medical University

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

Guangxi Medical University

China National Traditional Chinese Medicine Co. Ltd.

Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College

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