Effects of Three Kinds of Precursors on Cell Growth and Salidroside Content of Rhodiola sachalinensis in Cell Suspension Culture

WEI Xin-fang; ZHOU Bin; JIA Jing-ming

doi:10.13422/j.cnki.syfjx.2010.15.036

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Effects of Three Kinds of Precursors on Cell Growth and Salidroside Content of Rhodiola sachalinensis in Cell Suspension Culture

更新时间：2024-09-23

- Effects of Three Kinds of Precursors on Cell Growth and Salidroside Content of Rhodiola sachalinensis in Cell Suspension Culture
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 15, Pages: 83-86(2010)
- 作者机构：
  
  沈阳药科大学
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2010.15.036
  CLC： R284.1
- Published：2010
- 稿件说明：
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[1]魏欣方,周斌,贾景明.3种前体饲喂对高山红景天悬浮培养细胞中红景天苷的影响[J].中国实验方剂学杂志,2010,16(15):83-86.

WEI Xin-fang, ZHOU Bin, JIA Jing-ming. Effects of Three Kinds of Precursors on Cell Growth and Salidroside Content of Rhodiola sachalinensis in Cell Suspension Culture[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(15): 83-86.
[1]魏欣方,周斌,贾景明.3种前体饲喂对高山红景天悬浮培养细胞中红景天苷的影响[J].中国实验方剂学杂志,2010,16(15):83-86. DOI： 10.13422/j.cnki.syfjx.2010.15.036.

WEI Xin-fang, ZHOU Bin, JIA Jing-ming. Effects of Three Kinds of Precursors on Cell Growth and Salidroside Content of Rhodiola sachalinensis in Cell Suspension Culture[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(15): 83-86. DOI： 10.13422/j.cnki.syfjx.2010.15.036.

摘要

目的:研究前体饲喂对高山红景天细胞生长和红景天苷含量的影响。方法:在细胞培养起始期分别添加苯丙氨酸、肉桂酸和酪氨酸3种前体

在细胞生长的稳定期第14 d收获细胞

测定细胞干重、红景天苷含量、多酚氧化酶（PPO）和苯丙氨酸裂解酶（PAL）的活性。结果:苯丙氨酸的最佳添加质量浓度为20 mg·L-1

肉桂酸和酪氨酸的最佳添加质量浓度均为10 mg·L-1

此时红景天苷含量分别为2.65

3.50

3.21 mg·g-1。但高浓度的酪氨酸对细胞的生长有一定的抑制作用。结论:3种前体在合适的浓度下均能促进细胞中红景天苷的生物合成

并提高多酚氧化酶（PPO）、苯丙氨酸裂解酶（PAL）的活性

其中肉桂酸作用明显。

Abstract

Objective:To study the effect of precursor feeding on cell growth and salidroside content of Rhodiola sachalinensis A.Bor.Method:Three kinds of precursor of L-phenylalanine

cinnamic acid and tyrosine were added in the initial period of cell culture and cells were harvested to measure dry weight

salidroside content

polyphenol oxidase（PPO） and phenylalanine lyase（PAL） activity in the 14 d

i e in the stability phase of the cell growth curve.Result:The best concentrations of phenylalanine

cinnamic acid and tyrosine to be added were 20

10 mg·L-1

while salidroside content was 2.65

3.50

3.21 mg·g-1 respectively.But high concentration of tyrosine would restrain cell growth.Conclusion:Three kinds of precursor in appropriate concentration could all promote the biosynthesis of salidroside and increase polyphenol oxidase（PPO）

phenylalanine lyase（PAL） activity

among them

cinnamic acid did work obviously.

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Related Author

WEI Xin-fang

ZHOU Bin

JIA Jing-ming

XU Haoqun

ZHANG Wenjing

LOU Yuan

JIANG Yanan

YUAN Xin

Related Institution

Shandong University of Traditional Chinese Medicine（TCM）

Innovation Center of Classic TCM Formula

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Gansu Provincial Academic Institute for Medical Research， Gansu Provincial Cancer Hospital

Shandong Academy of Chinese Medicine

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