Effect of Shuanggen Qingnao Granules on Proliferation and Migration of Neural Stem Cells in Rat with Vascular Dementia

WU Hao-xin; LI Hui; YUE Xiao-jie; ZHEN Hong-yan

doi:10.13422/j.cnki.syfjx.2010.15.043

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Effect of Shuanggen Qingnao Granules on Proliferation and Migration of Neural Stem Cells in Rat with Vascular Dementia

更新时间：2024-09-23

- Effect of Shuanggen Qingnao Granules on Proliferation and Migration of Neural Stem Cells in Rat with Vascular Dementia
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 15, Pages: 110-113(2010)
- 作者机构：
  
  南京中医药大学脑病基础研究实验室
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2010.15.043
  CLC： R285.5
- Published：2010
- 稿件说明：
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[1]吴颢昕,李辉,岳晓杰,郑鸿燕.双根清脑颗粒对血管性痴呆模型大鼠神经干细胞增殖迁移的影响[J].中国实验方剂学杂志,2010,16(15):110-113+116.

WU Hao-xin, LI Hui, YUE Xiao-jie, et al. Effect of Shuanggen Qingnao Granules on Proliferation and Migration of Neural Stem Cells in Rat with Vascular Dementia[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(15): 110-113.
[1]吴颢昕,李辉,岳晓杰,郑鸿燕.双根清脑颗粒对血管性痴呆模型大鼠神经干细胞增殖迁移的影响[J].中国实验方剂学杂志,2010,16(15):110-113+116. DOI： 10.13422/j.cnki.syfjx.2010.15.043.

WU Hao-xin, LI Hui, YUE Xiao-jie, et al. Effect of Shuanggen Qingnao Granules on Proliferation and Migration of Neural Stem Cells in Rat with Vascular Dementia[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(15): 110-113. DOI： 10.13422/j.cnki.syfjx.2010.15.043.

摘要

目的:观察双根清脑颗粒对血管性痴呆（VD）模型大鼠神经干细胞（NSCs）增殖、迁移的影响

探讨双根清脑颗粒治疗VD的机理。方法:SPF级SD大鼠42只

随机分为双根清脑颗粒高、中、低剂量组

脑复康组、模型组、假手术组、正常组等7组

每组大鼠6只。采用注入同种血栓栓子方法造成VD模型

中药高、中、低剂量组分别以双根清脑颗粒36

4 g.kg-1 ig

脑复康组以0.108 g.kg-1 ig

给药容积均为1.5 mL.kg-1

模型组、假手术组和正常组以等量生理盐水ig。免疫组化法分别标记5-溴脱氧尿苷（BrdU）阳性细胞和微管相关蛋白（DCX）阳性细胞

显微镜下观察BrdU与DCX阳性细胞的表达。结果:双根清脑颗粒中、低剂量组、脑复康组大鼠皮质区与SGZ区BrdU阳性细胞数均明显增加（P<0.05）;双根清脑颗粒中、低剂量组SGZ区BrdU阳性细胞比脑复康组增加更为显著（P<0.01）。假手术组以及双根清脑颗粒中、低剂量组大鼠SGZ区DCX阳性细胞数明显增加（P<0.01）

高剂量组、脑复康组大鼠SGZ区DCX阳性细胞数亦显著增加（P<0.05）;双根清脑颗粒中、低剂量组大鼠SGZ区DCX阳性细胞数比脑复康组明显增加（P<0.01）。结论:双根清脑颗粒可促进VD模型大鼠脑内NSCs的增殖与迁移。

Abstract

Objective:To observe the effect of Shuanggen Qingnao granules on proliferation and migration of neural stem cells（NSCs） in model of rats with vascular dementia and its treatment mechanism for vascular dementia（VD）.Method:Forty two SPF level SD rats were randomly divided into large

medium

low-dose of Shuanggen Qingnao granules groups

piracetam group

model group

sham operation group

the normal group（n = 6

each）.VD rat model was established by slowly injecting the same kinds of thrombotic emboli to the catheter of the external carotid artery.5-bromo-2-deoxyuriding（BrdU） positive cells and doublecortin（DCX） positive cells were respectively marked by immunohistochemical method and their expressions were observed by microscope.Result: Compared with model group

whether in cerebral cortex or hippocampus

BrdU positive cells of medium

low-dose of Shuanggen Qingnao granules groups and piracetam group were significantly increased（P < 0.05）;compared with piracetam group

BrdU positive cells of medium

low-dose of Shuanggen Qingnao granules groups in cerebral hippocampus were also significantly increased（P < 0.01）.DCX-positive cells in rats’SGZ district of normal group

sham operation group and medium

low-dose of Shuanggen Qingnao granules groups were significantly increased（P <0.01）;compared with the model group

DCX-positive cells of large-dose of Shuanggen Qingnao granules group and piracetam group were also significantly increased（P < 0.05）;DCX-positive cells in rats’SGZ district of medium

low-dose of Shuanggen Qingnao granules groups compared with the piracetam group were obviously increased（P < 0.01）.Conclusion:Shuanggen Qingnao granules can promote the proliferation and migration of NSCs in inner brain of VD rats.

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Related Author

WU Hao-xin

LI Hui

YUE Xiao-jie

ZHEN Hong-yan

HOU Benchao

HE Zhijian

LIU Haiyun

LIN Qianxia

Related Institution

The First Affiliated Hospital of Nanchang University

Jiangxi Cancer Hospital

School of Chinese Medicine， Jiangxi University of Chinese Medicine

The First Clinical College， Anhui University of Chinese Medicine

The First Affiliated Hospital of Anhui University of Chinese Medicine

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