Effect of Bushen Wenyang Huayu on Expression of HIF-1α and GLUT-1 in Rat Model of Endometriosis

YUAN Xiao-qin1; BIAN Wen-hui2

doi:10.13422/j.cnki.syfjx.2010.15.050

您当前的位置：

首页 >

文章列表页 >

Effect of Bushen Wenyang Huayu on Expression of HIF-1α and GLUT-1 in Rat Model of Endometriosis

更新时间：2024-09-23

- Effect of Bushen Wenyang Huayu on Expression of HIF-1α and GLUT-1 in Rat Model of Endometriosis
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 15, Pages: 132-135(2010)
- 作者机构：
  
  1. 海南省人民医院
  2. 河北省中医院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2010.15.050
  CLC： R285.5
- Published：2010
- 稿件说明：
移动端阅览
[1]袁小琴,边文会.补肾温阳化瘀方对子宫内膜异位症模型大鼠内膜组织缺氧诱导因子-1α、葡萄糖转运因子-1表达的影响[J].中国实验方剂学杂志,2010,16(15):132-135.

YUAN Xiao-qin1, BIAN Wen-hui2. Effect of Bushen Wenyang Huayu on Expression of HIF-1α and GLUT-1 in Rat Model of Endometriosis[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(15): 132-135.
[1]袁小琴,边文会.补肾温阳化瘀方对子宫内膜异位症模型大鼠内膜组织缺氧诱导因子-1α、葡萄糖转运因子-1表达的影响[J].中国实验方剂学杂志,2010,16(15):132-135. DOI： 10.13422/j.cnki.syfjx.2010.15.050.

YUAN Xiao-qin1, BIAN Wen-hui2. Effect of Bushen Wenyang Huayu on Expression of HIF-1α and GLUT-1 in Rat Model of Endometriosis[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(15): 132-135. DOI： 10.13422/j.cnki.syfjx.2010.15.050.

摘要

目的:探讨补肾温阳化瘀方治疗内异症的可能作用机制。方法:选用SPF级雌性大鼠复制腹膜型内异症大鼠模型

将造模成功的大鼠分为4组:补肾温阳化瘀方高剂量组（16只）、低剂量组（16只）、丹那唑组（16只）、模型组（17只）

并设对照组（12只）。运用免疫组化（S-P）法

检测内异症模型大鼠治疗前、后异位内膜组织中缺氧诱导因子-1α（HIF-1α）、葡萄糖转运因子-1（GLUT-1）的表达

并比较治疗前后异位灶体积变化。结果:补肾温阳化瘀方高、低剂量组及丹那唑组治疗后子宫内膜异位灶体积较治疗前明显缩小（P<0.05）

且以补肾温阳化瘀方高剂量组最显著（P<0.01）;HIF-1α

GLUT-1在模型组异位内膜的表达与对照组在位内膜相比显著升高（P<0.01）;补肾温阳化瘀方高、低剂量组及丹那唑组均能够显著抑制HIF-1α

GLUT-1的高表达（P<0.01）

与对照组表达无明显差异

且以中药高剂量组作用效果最显著

低剂量组次之;丹那唑组与补肾温阳化瘀方高剂量组比较抑制效果有差异（P<0.05）。结论:补肾温阳化瘀方可能通过抑制HIF-1α与GLUT-1的表达达到治疗内异症的目的。

Abstract

Objective:To explore the mechanism of Bushen Wenyang Huayu（BSWYHY） on endometriosis（EMs）.Method: The peritoneal type endometriosis in SPF-grade female rats was established.They were then divided into 4 groups: BSWY high dose group（16）

BSWYHY low dose group（16）

danazol group（16）

model group（17）

and control group（12）.Immunohistochemistry was performed to examine the expression of HIF-1α and GLUT-1 in the ectopic endometrial tissues of the EMs model rats before and after treatment

and the volume change in endometriotic lesions before and after treatment was compared.Result:After treatment with high dose and low dose of BSWY and danazol

volumes of ectopic lesions were reduced obviously（P < 0.05）

and the volume of the high dose group demonstrated the most notable reduction（P < 0.01）.The expression of HIF-1α and GLUT-1 in ectopic EMs of the model group is significantly higher than that in eutopic endometrium in the control group（P < 0.01）.The BSWYHY high and low dose group and the danazol group were able to inhibit the high expression of HIF-1α and GLUT-1 substantially（P < 0.01）

with the high dose group as the best

followed by the low dose anddanazol group（P < 0.05）.Conclusion: BSWY can be an effective measure for reducing the high expression of HIF-1α and GLUT-1 for the treatment of EMs.

关键词

Keywords

references

Views

150

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Discussion on the Problem of Quality Control of Chinese Drugs

Influence and of Endometriosis Model Mouse’s Liquid of Abdominal Cavity PGF and Serum CAwhich Treated with Xiaozheng Decoction(XZD)

Fluorescence Probe, Detective Technology and its Application in Active Tracking of Traditional Chinese Macromolecules

Related Author

BIAN Wen-hui

YUAN Xiao-qin

Xie Ming

WANG Xin

ZHANG Jing-jing

JI Ling-shi

SUN Ming-e

XU Hui-jun

Related Institution

Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences

School of Pharmaceutical Sciences,Jilin University

The Morphology Lab,Experimental Research Center,China Academy of Chinese Medical Sciences

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰