Effect of Gengnianchun Formula on Hippocampal BDNF of Ovariectomized SD Rat

ZHAO Fan-gui 1; WANG Wen-jun1; ZHOU Wen-jiang 2; LI Da-jin1

doi:10.13422/j.cnki.syfjx.2010.15.052

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Effect of Gengnianchun Formula on Hippocampal BDNF of Ovariectomized SD Rat

更新时间：2024-09-23

- Effect of Gengnianchun Formula on Hippocampal BDNF of Ovariectomized SD Rat
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 15, Pages: 140-142(2010)
- 作者机构：
  
  1. 复旦大学附属妇产科医院
  2. 复旦大学实验动物部
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2010.15.052
  CLC： R285.5
- Published：2010
- 稿件说明：
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[1]赵凡桂,王文君,周文江,李大金.更年春方对卵巢切除大鼠海马中脑源性神经营养因子的影响[J].中国实验方剂学杂志,2010,16(15):140-142.

ZHAO Fan-gui 1, WANG Wen-jun1, ZHOU Wen-jiang 2, et al. Effect of Gengnianchun Formula on Hippocampal BDNF of Ovariectomized SD Rat[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(15): 140-142.
[1]赵凡桂,王文君,周文江,李大金.更年春方对卵巢切除大鼠海马中脑源性神经营养因子的影响[J].中国实验方剂学杂志,2010,16(15):140-142. DOI： 10.13422/j.cnki.syfjx.2010.15.052.

ZHAO Fan-gui 1, WANG Wen-jun1, ZHOU Wen-jiang 2, et al. Effect of Gengnianchun Formula on Hippocampal BDNF of Ovariectomized SD Rat[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(15): 140-142. DOI： 10.13422/j.cnki.syfjx.2010.15.052.

摘要

目的:研究中药更年春方（GNC）对卵巢切除大鼠海马中脑源性神经营养因子（brain-derived neurotrophic factor

BDNF）的调节作用。方法:选择10～12月龄SD雌性大鼠随机分为5组（假手术组

模型组

GNC 6.58 g·kg-1组

GNC 3.29 g·kg-1组

尼尔雌醇3.05×10-4 g.kg-1组）

切除双侧卵巢建立学习和记忆能力下降的模型

分别用高、低剂量GNC、尼尔雌醇和生理盐水ig 12周

行Morris水迷宫试验

取海马组织

real time PCR法测定海马BDNF mRNA的变化

免疫组化法测定海马CA3区BDNF蛋白的表达。结果:卵巢切除大鼠海马BDNF mRNA和BDNF蛋白水平下降

而高剂量更年春方和尼尔雌醇提高切除卵巢大鼠海马中BDNF mRNA和蛋白的表达。结论:更年春方可以提高卵巢切除大鼠海马中BDNF的表达

可能通过这个机制改善卵巢切除大鼠的学习记忆能力。

Abstract

Objective:To study the effect of Gengnianchun formula（GNC） on BDNF in hippocampus of ovariectomized SD rat.Method: Ten to twelve months old SD rats were randomized into mimic sham operation group

ovariectomized（OVX） group + NS

OVX + high-dose GNC group

OVX + low-dose GNC group and OVX + Nilestriol group.A little fat around ovary was cut in mimic sham operation group rats.Ovariectomy was operated in four other groups.These rats were fed with GNC

nilestriol or NS for 12 weeks respectively.The changes learning and memory were tested by Morris water maze（MWM）.The real time PCR was performed to determine the mRNA level of BDNF in right hippocampuses.And the immunohistochemistry was performed to detect the distribution of protein of BDNF in paraffin section of left hippocampus CA3.Result:Twelve weeks after ovariectomy

the scale of BDNF mRNA and protein decreased in ovariectomized rat

but rats fed with GNC or Nilestriol had higher scale of BDNF mRNA and protein.Conclusion:GNC may increase the BDNF mRNA and protein in hippocampus

and to improve the learning and memory ability for the ovx rats.

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Related Author

ZHAO Fan-gui

WANG Wen-jun

ZHOU Wen-jiang

LI Da-jin

SHI Xue-li

DU Zheng-cai

XIA Meng

ZHAO Xiao-fang

Related Institution

Heilongjiang University of Chinese Medicine

Graduate School of Heilongjiang University of Chinese Medicine

Institute of Basic Theory for Traditional Chinese Medicine，China Academy of Chinese Medical Sciences

TCM （Zhongjing） School， Henan University of Chinese Medicine

Ningxia Hui Autonomous Region Hospital of TCMe and Research Institute of TCM

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