Determination of Contents of Adenine Nucleotides and Energy Charge in Rat Liver Tissue Treated with Aconite Roots by HPLC

GAO Na; YANG Yong; WANG Shi-jun; RONG Rong; LIN Jian-jun; WANG Xiao-ping

doi:10.13422/j.cnki.syfjx.2010.15.062

您当前的位置：

首页 >

文章列表页 >

Determination of Contents of Adenine Nucleotides and Energy Charge in Rat Liver Tissue Treated with Aconite Roots by HPLC

更新时间：2024-09-23

- Determination of Contents of Adenine Nucleotides and Energy Charge in Rat Liver Tissue Treated with Aconite Roots by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 15, Pages: 172-175(2010)
- 作者机构：
  
  山东中医药大学
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2010.15.062
  CLC： R285
- Published：2010
- 稿件说明：
移动端阅览
[1]高娜,杨勇,王世军,容蓉,蔺建军,王小平.HPLC测定附子对大鼠肝组织中腺苷酸含量及能荷的影响[J].中国实验方剂学杂志,2010,16(15):172-175.

GAO Na, YANG Yong, WANG Shi-jun, et al. Determination of Contents of Adenine Nucleotides and Energy Charge in Rat Liver Tissue Treated with Aconite Roots by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(15): 172-175.
[1]高娜,杨勇,王世军,容蓉,蔺建军,王小平.HPLC测定附子对大鼠肝组织中腺苷酸含量及能荷的影响[J].中国实验方剂学杂志,2010,16(15):172-175. DOI： 10.13422/j.cnki.syfjx.2010.15.062.

GAO Na, YANG Yong, WANG Shi-jun, et al. Determination of Contents of Adenine Nucleotides and Energy Charge in Rat Liver Tissue Treated with Aconite Roots by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(15): 172-175. DOI： 10.13422/j.cnki.syfjx.2010.15.062.

摘要

目的:采用反相高效液相色谱（HPLC）技术

探讨中药附子对正常大鼠肝组织中三磷酸腺苷（ATP）

二磷酸腺苷（ADP）及一磷酸腺苷（AMP）含量及其对能荷（EC）水平的影响。方法:将20只Wister大鼠随机分为2组

分别灌胃附子水煎液和生理盐水4周

4周后麻醉处死

取肝组织进行反相高效液相色谱测定。结果:附子给药组大鼠较对照组大鼠肝脏中ATP含量明显升高

提高了70.3%

具有极显著性差异（P<0.01）

ADP

AMP的含量2组间无明显变化

总腺苷酸库、EC水平附子给药组与对照组比亦显著性升高（P<0.05）。结论:热性中药附子可以增加机体能量储备

能显著提高机体肝组织的ATP含量和EC水平

起到影响肝脏能量代谢的作用。

Abstract

Objective: To investigate the influence of traditional Chinese medicine aconite roots on the contents of adenosine triphosphate（ATP）

adenosine diphosphate（ADP） and adenosine monophosphate（AMP） in normal rats liver tissue and its impact on the energy charge by RP-HPLC.Method: 20 Wister rats were divided into two groups randomLy and were administered with decoction of aconite roots or saline respectively for four weeks.The contents of adenine nucleotides in rat liver tissues were analysed by RP-HPLC after four weeks.Result: The hepatic ATP in aconite roots treated group increased significantly by 70.3%（P < 0.01）

with high statistical significancy compared with that in control group

whereas the content of ADP and AMP showed no obvious differences between these two groups.The total adenylate library and energy charge（EC） levels in aconite roots treated group also increased significantly（P < 0.05） compared with that in the control group.Conclusion: Aconite roots

the hot natured Chinese herbal medicine

can boost the body energy reserves

increase the ATP content and EC level in liver tissue

and thus influence the hepatic energy metabolism.

关键词

Keywords

references

Views

556

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Determination of Glycyrrhizin in Weiwanshu Granule by HPLC

Determination of Icariin in Syrup by HPLC

双黄连含片中黄芩甙、绿原酸及连翘甙的含量测定

Determination of Puerarin in Gegen Qinlian Weiwan by HPLC

Related Author

GAO Na

YANG Yong

WANG Shi-jun

RONG Rong

LIN Jian-jun

WANG Xiao-ping

SONG Hong-jie

QUAN Shan-cong

Related Institution

广西中医药大学赛恩斯新医药学院,广西中医药大学,广西中医药大学赛恩斯新医药学院

成都中医药大学,九江学院,重庆市中药研究院重庆市中药资源重点实验室,重庆市中药研究院重庆市中药资源重点实验室,重庆市中药研究院重庆市中药资源重点实验室

Dongcheng District Institute for Drug Control

College of Pharmaceutical Science,Dali University,Dali

Shanxi Buchang Pharmaceutical Co.Ltd

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰