Rapid Screening Marine Active Substances by Accelerated Solvent Extraction Combined with Resazurin Assay

LIU Ping-huai; LIU Yang-yang; CHEN De-li; SHI Jie; HAO Zong-di

doi:10.13422/j.cnki.syfjx.2010.15.063

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Rapid Screening Marine Active Substances by Accelerated Solvent Extraction Combined with Resazurin Assay

更新时间：2024-09-23

- Rapid Screening Marine Active Substances by Accelerated Solvent Extraction Combined with Resazurin Assay
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 15, Pages: 176-179(2010)
- 作者机构：
  
  海南大学材料与化工学院海南优势资源化工材料应用技术教育部重点实验室
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2010.15.063
  CLC： R284.1
- Published：2010
- 稿件说明：
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[1]刘平怀,刘洋洋,陈德力,时杰,郝宗娣.ASE联合微孔板刃天青显色法快速筛选海洋抗菌活性物质[J].中国实验方剂学杂志,2010,16(15):176-179.

LIU Ping-huai, LIU Yang-yang, CHEN De-li, et al. Rapid Screening Marine Active Substances by Accelerated Solvent Extraction Combined with Resazurin Assay[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(15): 176-179.
[1]刘平怀,刘洋洋,陈德力,时杰,郝宗娣.ASE联合微孔板刃天青显色法快速筛选海洋抗菌活性物质[J].中国实验方剂学杂志,2010,16(15):176-179. DOI： 10.13422/j.cnki.syfjx.2010.15.063.

LIU Ping-huai, LIU Yang-yang, CHEN De-li, et al. Rapid Screening Marine Active Substances by Accelerated Solvent Extraction Combined with Resazurin Assay[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(15): 176-179. DOI： 10.13422/j.cnki.syfjx.2010.15.063.

摘要

目的:建立一种快速筛选和评价抗菌活性物质的方法

并将其应用于海洋抗菌活性物质的筛选。方法:在对生物样品的提取过程中引入加速溶剂萃取技术（ASE）

同时采用刃天青作为指示剂间接判断不同提取物样品的抗菌特性。结果:建立了一种快速筛选抗菌药物的方法

并发现白棘三列海胆、梅氏长海胆、面包海星、秀丽节蛇尾的乙酸乙酯提取物对实验菌生长均具有较强抑制作用。结论:该方法克服了常规的纸片琼脂扩散法、试管肉汤稀释法等作为抗菌药物筛选方法的不足

可使数量庞大的生物活性物质中抗菌药物的高通量筛选与评价成为可能。

Abstract

Objective: To establish a rapid screening and evaluating method for antibacterial active substance

and apply it to the screen of antibacterial marine active substance.Method: accelerated solvent extraction was applied during the active substance extraction procedure of biological samples

and then resazurin was used as a indicator to determine the antibacterial ability.Result: A rapid screening method for antibacterial medicine was worked out.What’s more

we found that the ethyl acetate extract of Tripnetistes gratilla

Echinometra mathaei

Culcita novaeguineae and Ophiarthrum elegans have relatively strong inhibition to the experimental bacteria.Conclusion: It is an applicable method to realize high-throughput screen and evaluation of antibacterial biological-active substance

and overcome the shortage of conventional methods such as conventional methods.

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HAO Zong-di

SHI Jie

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Yu WEI

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Related Institution

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