HPLC Determination of Gallic Acid and Quercetin in Different Parts of Archidendron clypearia

LI Xue-ling; LIU Li; ZHONG Reng; LI Sha-sha; WENG Li-dong; ZHANG Lu; LIU Qiang

doi:10.13422/j.cnki.syfjx.2010.18.011

您当前的位置：

首页 >

文章列表页 >

HPLC Determination of Gallic Acid and Quercetin in Different Parts of Archidendron clypearia

更新时间：2024-09-23

- HPLC Determination of Gallic Acid and Quercetin in Different Parts of Archidendron clypearia
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 18, Pages: 95-97(2010)
- 作者机构：
  
  南方医科大学中医药学院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2010.18.011
  CLC： R284
- Published：2010
- 稿件说明：
移动端阅览
[1]李雪玲,刘莉,钟棱,李沙沙,翁立冬,张璐,刘强.HPLC测定猴耳环不同部位中没食子酸和槲皮素的含量[J].中国实验方剂学杂志,2010,16(18):95-97.

LI Xue-ling, LIU Li, ZHONG Reng, et al. HPLC Determination of Gallic Acid and Quercetin in Different Parts of Archidendron clypearia[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(18): 95-97.
[1]李雪玲,刘莉,钟棱,李沙沙,翁立冬,张璐,刘强.HPLC测定猴耳环不同部位中没食子酸和槲皮素的含量[J].中国实验方剂学杂志,2010,16(18):95-97. DOI： 10.13422/j.cnki.syfjx.2010.18.011.

LI Xue-ling, LIU Li, ZHONG Reng, et al. HPLC Determination of Gallic Acid and Quercetin in Different Parts of Archidendron clypearia[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(18): 95-97. DOI： 10.13422/j.cnki.syfjx.2010.18.011.

摘要

目的:测定猴耳环药材不同部位没食子酸和槲皮素的含量

为合理开发利用猴耳环资源提供理论依据。方法:采用高效液相法

色谱条件:Kromosil C18色谱柱（4.6 mm×250 mm

5μm）

以乙腈-0.2%磷酸溶液梯度洗脱

流速1 mL.min-1

检测波长254 nm。结果:没食子酸和槲皮素的含量均是在叶中最高

幼枝中次之

茎干中含量最低

茎干中没有检测出槲皮素。结论:HPLC简便可行、重复性好

适用于猴耳环2种成分的同时测定

可用于猴耳环的质量控制。

Abstract

Objective:To determine gallic acid and quercetin from different parts of Archidendron clypearia

and to provide evidence for the rational development and utilization of A.clypearia.Method: HPLC method was used with Kromosil C18column（4.6 mm × 250 mm

5 μm）

and the mobile phases were acetonitrile and 0.2% phosphoric acid solution with the gradient elution.The flow rate was 1 mL.min-1 and the detection wavelength was 254 nm.Result: Both of the content of gallic acid and quercetin are highest in the leaves

lower in the new shoot and lowest in the shoot

even quercetin cannot be tested in the shoot.Conclusion: The method can be used to control the quality of Archidendron clypearia.It is simple

accurate

reproducible and suitable for simultaneous determination of two active constituents in Archidendron clypearia.

关键词

Keywords

references

Views

277

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

HPLC Determination of Gallic Acid and Quercetin in Different Parts of

Determination of Gallic acid, Salidroside, Tyrosol and -Coumaric Acid in Rhodiola Extract by HPLC

Determination of Total Tannins in Fruits of Tibetan Medicine from Different Areas

Analysis of HPLC Fingerprints and Determination of Gallic Acid and Ellagic Acid of Phyllanthi Fructus from

Analysis of Spectrum-effect Relationship of Antioxidant Activity of Sanguotang Based on Partial Least Square Method

Related Author

LIU Qiang

ZHANG Lu

WENG Li-dong

LI Sha-sha

ZHONG Reng

LIU Li

LI Xue-ling

ZHONG Yan

Related Institution

No data

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰