Determination of Multiple Active Components in Complex Prescription of Salvia miltiorrhiza by RP-HPLC

YOU Yan1; XU Guo-Liang2; ZHANG Qi-Yun2; TANG Xi-Lan2; ZHENG Qin2; YANG Ming2

doi:10.13422/j.cnki.syfjx.2010.18.029

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Determination of Multiple Active Components in Complex Prescription of Salvia miltiorrhiza by RP-HPLC

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- Determination of Multiple Active Components in Complex Prescription of Salvia miltiorrhiza by RP-HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 18, Pages: 50-53(2010)
- 作者机构：
  
  1. 南昌大学第四附属医院
  2. 江西中医学院现代中药制剂教育部重点实验室
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2010.18.029
  CLC： R286
- Published：2010
- 稿件说明：
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[1]游燕,徐国良,张启云,汤喜兰,郑琴,杨明.RP-HPLC同时测定复方丹参片中多种有效成分的含量[J].中国实验方剂学杂志,2010,16(18):50-53.

YOU Yan1, XU Guo-Liang2, ZHANG Qi-Yun2, et al. Determination of Multiple Active Components in Complex Prescription of Salvia miltiorrhiza by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(18): 50-53.
[1]游燕,徐国良,张启云,汤喜兰,郑琴,杨明.RP-HPLC同时测定复方丹参片中多种有效成分的含量[J].中国实验方剂学杂志,2010,16(18):50-53. DOI： 10.13422/j.cnki.syfjx.2010.18.029.

YOU Yan1, XU Guo-Liang2, ZHANG Qi-Yun2, et al. Determination of Multiple Active Components in Complex Prescription of Salvia miltiorrhiza by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(18): 50-53. DOI： 10.13422/j.cnki.syfjx.2010.18.029.

摘要

目的:采用高效液相色谱

切换波长-梯度洗脱法同时测定复方丹参制剂中9个有效成分的含量。方法:应用反相C18柱;以乙腈-0.1%磷酸溶液作为梯度洗脱流动相

流速1.0 mL.min-1

检测波长281

203

270 nm;柱温30℃。结果:9个成分的峰面积与浓度的线性关系良好（r>0.999 0）;回收率为94.87%～104.31%。结论:此方法可作为复方丹参制剂多成分质量控制的标准。

Abstract

Objective:To develop an HPLC method for determination of nine active components in complex prescription of salvia miltiorrhiza Bunge by wavelength switching.Method:This HPLC assay was performed on a reversed-phase C18 column with gradient elution of acetonitrile and 0.1% phosphoric acid solution

the flow rate was 1.0 mL.min-1

detecting wavelength was at 281

203 and 270 nm

and column temperature was 30 ℃.Result: Nine components were linear（r > 0.999 0） in the range of the test concentration

and the recoveries of the method were between 94.87%-104.31%

respectively.Conclusion: This method is suitable for the quality control of this commonly used complex prescription.

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Related Author

YOU Yan

XU Guo-liang

ZHANG Qi-yun

TANG Xi-lan

ZHENG Qin

YANG Ming

Hong-xing MA

Long-fei LIN

Related Institution

Jilin Agricultural University

Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences

Chengde Medical University

Jilin Huakang Pharmaceutical Co. Ltd.

The First Clinical Medical College of Guangzhou University of Chinese Medicine

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