Effects of Wogonin on Proliferation and Cell Cycle of SPC-A-1 Cells

SUN Jian1; ZHANG Yang1; WEN Qing-hui3; ZHANG Zheng-yao1; ZHOU Qiu-li1

doi:10.13422/j.cnki.syfjx.2010.18.034

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Effects of Wogonin on Proliferation and Cell Cycle of SPC-A-1 Cells

更新时间：2024-09-23

- Effects of Wogonin on Proliferation and Cell Cycle of SPC-A-1 Cells
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 18, Pages: 169-172(2010)
- 作者机构：
  
  1. 吉林大学药学院
  2. 中国中医科学院医学实验中心
  3. 国家食品药品监督管理局药品评价中心
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2010.18.034
  CLC： R285.5
- Published：2010
- 稿件说明：
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[1]孙健,张扬,温庆辉,张郑瑶,周秋丽.汉黄芩素对人肺腺癌SPC-A-1细胞增殖和周期的影响[J].中国实验方剂学杂志,2010,16(18):169-172.

SUN Jian1, ZHANG Yang1, WEN Qing-hui3, et al. Effects of Wogonin on Proliferation and Cell Cycle of SPC-A-1 Cells[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(18): 169-172.
[1]孙健,张扬,温庆辉,张郑瑶,周秋丽.汉黄芩素对人肺腺癌SPC-A-1细胞增殖和周期的影响[J].中国实验方剂学杂志,2010,16(18):169-172. DOI： 10.13422/j.cnki.syfjx.2010.18.034.

SUN Jian1, ZHANG Yang1, WEN Qing-hui3, et al. Effects of Wogonin on Proliferation and Cell Cycle of SPC-A-1 Cells[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(18): 169-172. DOI： 10.13422/j.cnki.syfjx.2010.18.034.

摘要

目的:研究汉黄芩素对人肺腺癌SPC-A-1细胞增殖和周期的影响。方法:以不同浓度的汉黄芩素作用于SPC-A-1细胞

检测细胞抑制率、凋亡及细胞周期。结果:MTT法测得汉黄芩素的IC50为24.7 mg.L-1

能够明显抑制SPC-A-1细胞的集落形成能力。汉黄芩素作用于SPC-A-1细胞后

荧光倒置显微镜下观察到细胞凋亡的形态学改变。Annexin V-FITC/PI双标法证实汉黄芩素可以诱导SPC-A-1细胞凋亡

并具有剂量依赖性。流式细胞仪结果显示

不同浓度汉黄芩素使SPC-A-1细胞周期出现显著的变化

20 mg.L-1汉黄芩素可将周期阻滞在G0/G1期

40 mg.L-1汉黄芩素则使细胞周期堆积在S期

并且随着给药剂量的增加

凋亡峰显著升高。结论:汉黄芩素能明显抑制SPC-A-1细胞增殖

诱导细胞凋亡

并对细胞G0/G1和S期具有阻滞效应

具有抗肿瘤作用。

Abstract

Objective:To investigate the effect of wogonin on the proliferation and cell cycle of the SPC-A-1 cell line.Method: The cultured SPC-A-1 cells were treated with different concentrations of wogonin.The proliferation of SPC-A-1 cells was detected by MTT method.Cell apoptosis and cell cycle were detected by AO + EB straining and flow cytometry.Result:After wogonin administration

SPC-A-1 cells proliferation inhibition ratio and apoptosis ratio increased significantly in a dose-dependent manner.AO + EB straining showed partial cells presented characteristic morphological changes of apoptosis.SPC-A-1 cells showed G0 /G1 phase blockage when the concentration of wogonin was 10

20 mg.L-1

and S phase blockage appeared when the concentration of wogonin was 30

40 mg.L-1.Conclusion:Wogonin can inhibit the proliferation of SPC-A-1 cells and induce apoptosis

also possess the effect of G0 /G1 and S phase blockage and potential antitumor effect.

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Related Author

SUN Jian

ZHANG Yang

WEN Qing-hui

ZHANG Zheng-yao

ZHOU Qiu-li

WU Yao-song

MA Jun-hua

CHEN Yu-long

Related Institution

Hebei Key Laboratory of Traditional Chinese Medicine（TCM） Research on Cardiocerebrovascular Disease，TCM Processing Technology Innovation Center of Hebei Province，College of Pharmacy， Hebei University of Chinese Medicine

Affiliated Hospital of Shandong University of Traditional Chinese Medicine（TCM）

Shandong University of TCM

School of Chinese Materia Medica， Beijing University of Chinese Medicine

School of Life Sciences，Beijing University of Chinese Medicine

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