Objective:To establish a determination method for quality control of Rougan capsule.Method: The contents of salvianolic acid B

curcumin

demethoxycurcumin and bisdemethoxycurcumin in Rougan capsule were determined by HPLC-DAD.These compounds were analyzed with a Apollo C18 column（4.6 mm × 250 mm

5 μm） by gradient elution using 4% acetic acid-acetonitrile as the mobile phase

the flow rata was 1.0 mL.min-1

the column temperature was maintained at 30 ℃ and the wavelength was detected at 286 and 426 nm.The content of tauroursodeoxycholic acid in Rougan capsule was determined by HPLC-ELSD.It was analyzed with a Apollo C18 column（4.6 mm × 250 mm

5 μm） by isocratic elution using acetonitrile-acetic acid-ammonium acetate solution（pH 4.5） as the mobile phase

the flow rate was 0.8 mL.min-1

the temperature of the drift tube was set at 80℃

the pressure of nebulizing gas（air） was at 35 psi

the temperature of the atomizer was at 36 ℃.Result: All these compounds were linear in the range of 0.148 0-1.850

0.125 6-1.570

0.043 2-0.540 0

0.033 6-0.420 0 and 0.972 0-12.15 μg（r = 0.999 3-0.999 9）

respectively.The RSDs of the precision

reproducibility and stability test were less than 3%;the average recovery of the method was in the range of 97.8%-101.7%.Conclusion: The established method is simple

accurate

and repeatable and can be used for quality control of Rougan capsule.