REN Jie, LIU Guang-bin, ZOU Gan-peng, et al. Determination of Matrine in Shuyin Lotion by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(18): 76-77.
REN Jie, LIU Guang-bin, ZOU Gan-peng, et al. Determination of Matrine in Shuyin Lotion by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(18): 76-77. DOI: 10.13422/j.cnki.syfjx.2010.18.043.
Determination of Matrine in Shuyin Lotion by HPLC
摘要
目的:建立舒阴洗液中苦参碱的含量测定方法。方法:采用高效液相色谱法
Inertsil ODS-SP色谱柱(4.6 mm×150 mm
5μm)
流动相乙腈-水-三乙胺(3∶97∶0.2
用磷酸调pH 3.5)
流速1.0 mL.min-1
检测波长220 nm。结果:苦参碱在3.9~117.0μg呈良好的线性关系(r=0.999 9)
平均回收率为101.69%
RSD 1.84%(n=9)。结论:本法简便、快速、准确
可用于该制剂的质量控制。
Abstract
Objective:To establish a method for determination of matrine in Shuyin Lotion.Method:The matrine content was determined by HPLC on inertsil ODS-SP column(4.6 mm × 150 mm
5 μm) with a mobile phase of acetonitrile-water-triethylamine(3∶ 97∶ 0.2
adjusted to pH 3.5 with phosphoric acid)
the flow rate was 1.0 mL.min-1
and the detection wavelength was set at 220 nm.Result: Matrine was linear in range of 3.9-117.0 μg(r =0.999 9).The average recovery was 101.69% with RSD of 1.84%(n =9).Conclusion: The method is simple
quick and accurate.It can be used for the quality control of Shuyin lotion.
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Related Author
REN Jie
LIU Guang-bin
ZOU Gan-peng
LIU Xiao-shuan
ZHANG Yu-bo
WANG Yan
MAO Yan
HE Jin-hua
Related Institution
Beijing Key Laboratory of Traditional Chinese Medicine(TCM) Quality Evaluation, Beijing University of Chinese Medicine
School of TCM, Beijing University of Chinese Medicine
National Institutes for Food and Drug Control
National Engineering Laboratory for Quality Control Technology of Chinese Materia Medica, Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences