LI Feng-jie, YAO Guang-tao, JIN Ruo-min, et al. Hepatotoxicity of Rats Induced by Radix et Rhizoma Sophorae Tonkinensis[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(18): 190-192.
LI Feng-jie, YAO Guang-tao, JIN Ruo-min, et al. Hepatotoxicity of Rats Induced by Radix et Rhizoma Sophorae Tonkinensis[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(18): 190-192. DOI: 10.13422/j.cnki.syfjx.2010.18.050.
Hepatotoxicity of Rats Induced by Radix et Rhizoma Sophorae Tonkinensis
Objective:To detect the hepatic toxicity after giving large dose of Radix et Rhizoma Sophorae Tonkinensis(RRST) to rats for a long time.Method: The rats were divided into 3 groups(control group
12 g.kg-1 drug group
20 g.kg-1 drug group) and given the drug for 26 days.During the days
the rats’behave and weights were observed.After giving the drug for the last time
the rats were anaesthetized by 25% Urethane and the rats’blood was extracted from aorta abdominalis
then liver function was detected by using the blood serum.The rats’livers were taken and weighed
after that
the viscera index was calculated.According to the demand of electron microscope
hepatic tissues were fixed and ultrastructures were detected.Biochemical indicators such as ALT
AST
ALP
TBiL
TP
GLO and ALB were detected by using automatic analyzer.Result: After giving RRST for 10 days
the activity of the rats in drug group decreased
what’s more
the rats had general fatigue and the amount their excrement and urine increased.After giving drug for 26 days
compared with normal group
the ALT and viscera index increased in drug group(P < 0.01 or P < 0.05)
the TP
ALB decreased in drug group(P < 0.01 or P < 0.05);Through the electron microscope
the results showed that in RRST group
the shape of nucleus changed
nucleus had the tendency of swelling
nuclear membranes shrunk
bile capillaries enlarged and smooth surfaced endoplasmic reticulum enlarged slightly.Conclusion: RRST can cause hepatic toxicity in rats by intragastric administration and the toxicity will increase if the dose of drug is added.