Experimental Study of Biyuanshu Influencing IL-8 and TNF-α mRNA Expression in Rabbits of Chronic Rhinosinusitis

LI Hui1; ZHU Tian-min2

doi:10.13422/j.cnki.syfjx.2010.18.051

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Experimental Study of Biyuanshu Influencing IL-8 and TNF-α mRNA Expression in Rabbits of Chronic Rhinosinusitis

更新时间：2024-09-23

- Experimental Study of Biyuanshu Influencing IL-8 and TNF-α mRNA Expression in Rabbits of Chronic Rhinosinusitis
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 18, Pages: 193-197(2010)
- 作者机构：
  
  1. 成都大学医护学院
  2. 成都中医药大学针推学院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2010.18.051
  CLC： R285.5
- Published：2010
- 稿件说明：
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[1]李辉,朱天民.鼻渊舒口服液对兔慢性鼻-鼻窦炎模型鼻窦黏膜IL-8,TNF-α mRNA表达的影响[J].中国实验方剂学杂志,2010,16(18):193-197.

LI Hui1, ZHU Tian-min2. Experimental Study of Biyuanshu Influencing IL-8 and TNF-α mRNA Expression in Rabbits of Chronic Rhinosinusitis[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(18): 193-197.
[1]李辉,朱天民.鼻渊舒口服液对兔慢性鼻-鼻窦炎模型鼻窦黏膜IL-8,TNF-α mRNA表达的影响[J].中国实验方剂学杂志,2010,16(18):193-197. DOI： 10.13422/j.cnki.syfjx.2010.18.051.

LI Hui1, ZHU Tian-min2. Experimental Study of Biyuanshu Influencing IL-8 and TNF-α mRNA Expression in Rabbits of Chronic Rhinosinusitis[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(18): 193-197. DOI： 10.13422/j.cnki.syfjx.2010.18.051.

摘要

目的:观察鼻渊舒口服液对兔慢性鼻窦炎（CRS）模型鼻窦黏膜IL-8

TNF-αmRNA表达的影响

探索鼻渊舒口服液治疗CRS的可能机制。方法:取新西兰大白兔60只

适应性喂养1周后

随机分为正常组、模型组、假手术组、鼻渊舒组、西药治疗组

每组12只

建立CRS模型;鼻渊舒组、西药治疗组分别给予鼻渊舒口服液1.5 mL.kg-1d-1

克拉霉素25 mg.kg-1.d-1

共14 d

处死后取鼻窦黏膜组织HE染色光镜观察

实时定量PCR法检测鼻窦黏膜IL-8

TNF-αmRNA表达。结果:模型组鼻窦黏膜呈慢性炎症表现

黏膜炎细胞浸润

上皮细胞、腺体和杯状细胞明显增生;鼻窦黏膜IL-8

TNF-αmRNA表达较正常组显著增高（P<0.01）。经鼻渊舒治疗后鼻窦黏膜上皮修复较好

炎细胞浸润、腺体和杯状细胞增生不明显;鼻窦黏膜IL-8

TNF-αmRNA表达较模型组显著降低（P<0.01）

与正常组比较无显著差异。结论:鼻渊舒口服液治疗CRS的机制可能与调控鼻窦黏膜IL-8

TNF-αmRNA表达有关。

Abstract

Objective:To observe IL-8 and TNF-α mRNA expression of rabbit sinus mucosa of Biyuanshu treatment of chronic rhinosinusitis（CRS） model

and explore the possible mechanism of Biyuanshu treatment of CRS.Method: Sixty new Zealand rabbits fed one week after adaptation and were randomLy divided into normal group

model group

sham operation group

Biyuanshu group

western medicine group

12 in each group.Firstly CRS model have established

then Biyuanshu group and western medicine group were given 1.5 mL.kg-1 of BiYuanShu or 25 mg.kg-1of clarithromycin every day in14 days

finally they were sacrificed to take sinus mucosa

HE staining were observed by light microscopy and IL-8 and TNF-α mRNA expression of sinus mucosa were detected by real-time quantitative PCR.Result: In model group

the sinus mucosa showed chronic inflammation and mucosal infiltration of inflammatory cells

and epithelial cells and glands and hyperplasia of goblet cells were found

IL-8 and TNF-α mRNA expression of sinus mucosal were higher than normal group significantly（P < 0.01）.In Biyuanshu group

sinus mucosa have good repair

and inflammatory cells and glands and goblet cell hyperplasia were not obvious

IL-8 and TNF-α mRNA expression of sinus mucosal were lower than model groupsignificantly（P < 0.01）

Biyuanshu group and normal group have no significant difference.Conclusion: The mechanism of Biyuanshu treatment of CRS may be related to regulation of IL-8 and TNF-α mRNA expression of sinus mucosal.

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Related Author

ZHU Tian-min

LI Hui

Yuan LU

Ting LIU

Jie PAN

Chun-hua LIU

Yong-jun LI

Yong-lin WANG

Related Institution

State Key Laboratory of Functions and Applications of Medicinal Plants， Guizhou Provincial Key Laboratory of Pharmaceutics， Engineering Research Center for the Development and Application of Ethnic Medicine and Traditional Chinese Medicine （Ministry of Education）， School of Pharmacy， Guizhou Medical University

Shandong College of Traditional Chinese Medicine

Guangzhou University of Chinese Medicine

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