Verification of Methodology on Microbial Limit Test for Shenrong Jianyaojiu

WANG Yu1; MAO De-fa1; QIN Kun-ming1; CAI Bao-chang1

doi:10.13422/j.cnki.syfjx.2010.18.052

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Verification of Methodology on Microbial Limit Test for Shenrong Jianyaojiu

更新时间：2024-09-23

- Verification of Methodology on Microbial Limit Test for Shenrong Jianyaojiu
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 18, Pages: 198-201(2010)
- 作者机构：
  
  1. 南京海昌中药集团
  2. 南京中医药大学国家教育部中药炮制规范化及标准化工程研究中心
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2010.18.052
  CLC： R286
- Published：2010
- 稿件说明：
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[1]王玉,毛德法,秦昆明,蔡宝昌.参蓉健腰酒微生物限度检查方法学的验证[J].中国实验方剂学杂志,2010,16(18):198-201.

WANG Yu1, MAO De-fa1, QIN Kun-ming1, et al. Verification of Methodology on Microbial Limit Test for Shenrong Jianyaojiu[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(18): 198-201.
[1]王玉,毛德法,秦昆明,蔡宝昌.参蓉健腰酒微生物限度检查方法学的验证[J].中国实验方剂学杂志,2010,16(18):198-201. DOI： 10.13422/j.cnki.syfjx.2010.18.052.

WANG Yu1, MAO De-fa1, QIN Kun-ming1, et al. Verification of Methodology on Microbial Limit Test for Shenrong Jianyaojiu[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(18): 198-201. DOI： 10.13422/j.cnki.syfjx.2010.18.052.

摘要

目的:建立参蓉健腰酒的微生物限度检查方法。方法:采用薄膜过滤法测定参蓉健腰酒对验证菌株的回收率

并对控制菌检查法进行验证。结果:确认此法可以用于参蓉健腰酒的微生物限度检查。在3次独立的平行试验中

5株试验菌回收率均大于70%

符合验证要求。控制菌（大肠埃希菌）采用常规法可检出。结论:该方法消除了样品的抑菌性

可用于该品种的微生物限度检查。

Abstract

Objective:To establish a method for the microbial limit test of Shenrong Jianyaojiu.Method: The recovery rates of tested strains treated by Shenrong Jianyaojiu were determined by membrane filtration method.The test method of the control bacteria was verified.Result: In the parallel experiment of 3 indendence

5 tested strains recovery rate all more than 70%.This method can be adopted in bacterial strain control of Shenrong Jianyaojiu.Routine test method can be test for control bacterias（Escherichia coli）.Conclusion: It can eliminate the antimicrobial properties

and it can be used for the microbial limit test for Shenrong Jianyaojiu.

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