Study on Processing Methods of Fructus Forsythiae

ZHANG Shu-rong; PEI Xiang-ping; LIANG Xue-wei; HUANG Xuan; HUANG Can-lin

doi:10.13422/j.cnki.syfjx.2010.18.061

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Study on Processing Methods of Fructus Forsythiae

更新时间：2024-09-23

- Study on Processing Methods of Fructus Forsythiae
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 18, Pages: 33-35(2010)
- 作者机构：
  
  山西中医学院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2010.18.061
  CLC： R283.6
- Published：2010
- 稿件说明：
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[1]张淑蓉,裴香萍,梁学伟,黄轩,黄灿林.青翘炮制方法的研究[J].中国实验方剂学杂志,2010,16(18):33-35+38.

ZHANG Shu-rong, PEI Xiang-ping, LIANG Xue-wei, et al. Study on Processing Methods of Fructus Forsythiae[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(18): 33-35.
[1]张淑蓉,裴香萍,梁学伟,黄轩,黄灿林.青翘炮制方法的研究[J].中国实验方剂学杂志,2010,16(18):33-35+38. DOI： 10.13422/j.cnki.syfjx.2010.18.061.

ZHANG Shu-rong, PEI Xiang-ping, LIANG Xue-wei, et al. Study on Processing Methods of Fructus Forsythiae[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(18): 33-35. DOI： 10.13422/j.cnki.syfjx.2010.18.061.

摘要

目的:确定青翘的最佳炮制方法。方法:采用HPLC直接测定

Alltima C18色谱柱（4.6 mm×250 mm

5μm）

甲醇-水梯度洗脱

检测波长280 nm

室温

流速0.8 mL.min-1。结果:连翘酯苷的回归方程为Y=959 631.703 1X+250 467.648 4

r=0.999 4

线性范围1.5～15μg;连翘苷的回归方程为Y=883 203.205 8X-9 047.738 3

r=0.999 4

线性范围0.145～1.45μg;连翘酯苷和连翘苷的加样回收率分别为97.9%

99.2%

RSD分别为2.30%

2.27%（n=6）。青翘药材蒸制20～30 min

连翘酯苷和连翘苷的含量最高。结论:该测定方法准确、可靠

重复性好

可用于连翘中连翘酯苷和连翘苷的含量测定;青翘的炮制以蒸制20～30 min为宜。

Abstract

Objective:To determine the optimal processing method for Fructus Forsythiae.Method: HPLC was applied on the determination with Alltima C18 column（4.6 mm × 250 mm

5 μm） and methanol-water as mo-bile phase by a gradient elution program at a flow rate of 0.8 mL.min-1.The detection wavelength was at 280 nm and column temperature was at room temperature.Result: The regression equation of Forsythiaside wasY = 959 631.703 1X + 250 467.648 4

r = 0.999 4

Linear range was 1.5-15 μg

The regression equation of phillyrin was Y = 883 203.205 8X-9 047.738 3

r = 0.999 4

Linear range was 0.145-1.45 μg.The average recovery of Forsythiaside and Phillyrin was 97.9% with RSD 2.30% and 99.2% with RSD 2.27%（n = 6） respectively.Content of the Forsythiaside and Phillyrin was in the maximum yield with the processing methods of steaming for 20-30 min steaming.Conclusion:The method was accurate and repeatable

can be applied to the quality control of Fructus Forsythiae.The optimal processing method of Fructus Forsythiae was steaming for 20-30 min.

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Related Author

ZHANG Shu-rong

PEI Xiang-ping

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HUANG Can-lin

LU Li

YANG Yichen

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Related Institution

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School of Pharmacy，Hanzhong Vocational and Technical College

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National Resource Center for Chinese Materia Medica，China Academy of Chinese Medical Sciences

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