Inhibition of Jianpi Jiedu Recipe on Angiogenesis in Helicobacter pylori-induced Gastric Cancer

LIU Ning-ning; WANG Yan; ZHOU Li-hong; ZHAO Cheng-gen; WU Dong-hui; FAN Zhong-ze; LI Qi

doi:10.13422/j.cnki.syfjx.2011.01.002

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Inhibition of Jianpi Jiedu Recipe on Angiogenesis in Helicobacter pylori-induced Gastric Cancer

更新时间：2024-09-23

- Inhibition of Jianpi Jiedu Recipe on Angiogenesis in Helicobacter pylori-induced Gastric Cancer
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 1, Pages: 88-92(2011)
- 作者机构：
  
  上海中医药大学附属普陀医院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2011.01.002
  CLC： R273
- Published：2011
- 稿件说明：
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[1]刘宁宁,王炎,周利红,赵成根,吴东辉,范忠泽,李琦.健脾解毒方对幽门螺杆菌诱发胃癌血管新生的抑制研究[J].中国实验方剂学杂志,2011,17(01):88-92+95.

LIU Ning-ning, WANG Yan, ZHOU Li-hong, et al. Inhibition of Jianpi Jiedu Recipe on Angiogenesis in Helicobacter pylori-induced Gastric Cancer[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(1): 88-92.
[1]刘宁宁,王炎,周利红,赵成根,吴东辉,范忠泽,李琦.健脾解毒方对幽门螺杆菌诱发胃癌血管新生的抑制研究[J].中国实验方剂学杂志,2011,17(01):88-92+95. DOI： 10.13422/j.cnki.syfjx.2011.01.002.

LIU Ning-ning, WANG Yan, ZHOU Li-hong, et al. Inhibition of Jianpi Jiedu Recipe on Angiogenesis in Helicobacter pylori-induced Gastric Cancer[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(1): 88-92. DOI： 10.13422/j.cnki.syfjx.2011.01.002.

摘要

目的:探讨健脾解毒方在幽门螺杆菌（Helicobacter pylori

H.pylori）长期感染诱发C57BL/6小鼠胃癌过程中对微血管密度（microvessel density

MVD）和血管新生相关基因的调控作用

为其治疗H.pylori感染相关胃病提供实验依据。方法:采用H.pylori标准株SS1 ig的方法建立H.pylori感染C57BL/6小鼠诱发胃癌动物模型

分为对照组、模型组、健脾解毒方低剂量组和健脾解毒方高剂量组。72周时

免疫组化法检测各组小鼠胃黏膜MVD的变化;实时荧光定量PCR（RFQ-PCR）法和免疫组化法检测各组小鼠胃黏膜血管内皮生长因子（vascular endothelial growth factor

VEGF）、血管生成素-2（angiopoietin-2

Ang-2）及其受体Tie-2 mRNA和蛋白的表达。结果:对照组、模型组、健脾解毒方低、高剂量组胃黏膜MVD分别为（2.50±1.54）

（18.56±2.62）

（14.61±3.60）

（7.39±1.75）个/视野。模型组MVD较对照组明显增高

P<0.01;健脾解毒方低、高剂量组MVD较模型组显著降低

P<0.01。模型组VEGF

Ang-2

Tie-2 mRNA表达较对照组都明显增加

P<0.01;健脾解毒方低、高剂量组均可显著降低三者的表达

并成一定的剂量依赖效应。结论:H.pylori诱发C57BL/6小鼠胃癌过程中

可增加胃黏膜MVD

促进VEGF

Ang-2

Tie-2的表达

在H.pylori诱导的胃癌发生中起到促进作用;健脾解毒方可减少MVD

抑制VEGF

Ang-2

Tie-2的表达

这可能是其防治胃癌的重要机制之一。

Abstract

Objective: To investigate the regulatory effects of Jianpi Jiedu Recipe on microvessel density（MVD） and angiogenesis-related genes in the course of Helicobacter pylori（H.pylori） long-term infection induced gastric adenocarcinoma of C57BL /6 mice

and provide experimental basis for Jianpi Jiedu Recioe treatment of the H.pylori-related gastropathy.Method: C57BL /6 mouse gastric cancer model induced by H.pylori infected was established by gavage with H.pylori standard strain SS1

and mice were divided into 4 groups: Control（Cont） group-model（Mod）group and low-dose（LD）and high-dose（HD）Jianpi Jiedu Recioe group.After 72 weeks

MVD in gastric mucosa of each group was detected with Evision immunohistochemical method.Vascular endothelial growth factor（VEGF）

angiopoietin-2（Ang-2） and Ang-2 type 2 receptor（Tie-2） mRNA and protein expressionwere evaluated by real-time fluorogenic quantitative polymerase chain reaction（RFQ-PCR） and immunohistochemical method.Result: Pathological examination demonstrated that at the 72 th week

the mice gastric mucosa MVD were 2.50 ± 1.54

18.56 ± 2.62

14.61 ± 3.60 and 7.39 ± 1.75 in Cont

Mod

LD and HD group respectively.The MVD in Mod group was obviously increased than that in Cont group

P < 0.01;The MVD were significantly decreased in LD and HD Jianpi Jiedu Recioe group

compared with Mod group

P < 0.05

P < 0.01.FRQ-PCR suggested that the expression of VEGF

Ang-2

Tie-2 mRNA and protein in Mod group were obviously increased than those in Cont group

P < 0.01;However both the LD and HD Jianpi Jiedu Recioe could significantly reduce their expression in a dose-dependent manner.Conclusion: H.pylori could increase MVD of gastric mucosa

and induce VEGF

Ang-2 and Tie-2 expression in the course of H.pylori infection-induced gastric adenocarcinoma of C57BL /6 mice

which might play an important role in the incidence of H.pylori-induced gastric cancer.Jianpi Jiedu Recipe could decrease MVD

and inhibit VEGF

Ang-2 and Tie-2 expression

which might be one of the important mechanisms of prevention and treatment of gastric cancer.

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Related Author

LIU Ning-ning

WANG Yan

ZHOU Li-hong

ZHAO Cheng-gen

WU Dong-hui

FAN Zhong-ze

LI Qi

WU Qiong

Related Institution

Laboratory Center,Putuo Hospital Affiliated to Shanghai University of Traditional Chinese Medicine

Department of Oncology,Putuo Hospital Affiliated to Shanghai University of Traditional Chinese Medicine

Graduate School， Hebei University of Chinese Medicine

Hebei Provincial Hospital of Traditional Chinese Medicine

Hebei Key Laboratory of Turbidity Toxin Syndrome

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