Optimization of Water-extracting and Alcohol-precipitating Conditions of Total Polysaccharides of Yupingfeng

ZHANG Li-ming1; ZHU Li1; LI Xue1; GAO Wen-yuan2

doi:10.13422/j.cnki.syfjx.2011.01.019

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Optimization of Water-extracting and Alcohol-precipitating Conditions of Total Polysaccharides of Yupingfeng

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- Optimization of Water-extracting and Alcohol-precipitating Conditions of Total Polysaccharides of Yupingfeng
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 1, Pages: 24-26(2011)
- 作者机构：
  
  1. 天津科技大学生物工程学院工业微生物教育部重点实验室
  2. 天津大学药物科学与技术学院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2011.01.019
  CLC： R284.2
- Published：2011
- 稿件说明：
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[1]张黎明,朱丽,李雪,高文远.玉屏风总多糖水提醇沉工艺条件优化[J].中国实验方剂学杂志,2011,17(01):24-26.

ZHANG Li-ming1, ZHU Li1, LI Xue1, et al. Optimization of Water-extracting and Alcohol-precipitating Conditions of Total Polysaccharides of Yupingfeng[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(1): 24-26.
[1]张黎明,朱丽,李雪,高文远.玉屏风总多糖水提醇沉工艺条件优化[J].中国实验方剂学杂志,2011,17(01):24-26. DOI： 10.13422/j.cnki.syfjx.2011.01.019.

ZHANG Li-ming1, ZHU Li1, LI Xue1, et al. Optimization of Water-extracting and Alcohol-precipitating Conditions of Total Polysaccharides of Yupingfeng[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(1): 24-26. DOI： 10.13422/j.cnki.syfjx.2011.01.019.

摘要

目的:优化玉屏风总多糖（YTP）的水提醇沉工艺条件。方法:以玉屏风总多糖得率为考察指标

采用正交试验法对YPT水提醇沉工艺的影响因素料液比、提取时间、醇沉浓度进行优化。结果:水提醇沉提取玉屏风总多糖的最佳提取工艺为:料液比1∶12

在90℃下浸提1.5 h

90%乙醇沉淀可使YTP得率达到4.31%。结论:该提取工艺可行

实验结果为确定玉屏风总多糖的水提醇沉工艺提供了实验依据。

Abstract

Objective:To seek out the optimal water-extracting and alcohol-precipitating technology of total polysaccharides of Yupingfeng（YTP）.Method: Using the yield of YTP as assessment index

three factors were studied by orthogonal design

including ratio of material（g） to extracting solvent（mL）

extracting time and ethanol concentration of precipitating polysaccharide.Result: The yield of YTP was 4.31% by the optimum process conditions listed as follows: ratio of material to extracting solvent was 1∶ 12

extracting time when the temperature was kept at 90 ℃ as 1.5 h;and the volume fraction of ethanol was 90%.Conclusion: These extraction process is feasible

and the experiment result can provide basic and instruction data for the ascertainment of water extraction technology of YTP.

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Related Author

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Related Institution

Faculty of Medicine,Beihua University

Henan University of Traditional Chinese Medicine

Zhoukou Hospital of Traditional Chinese Medicine

Chinese People’s Liberation Army 150 Central Hospital,Louyang

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