ZHANG Li, CHEN Ting, CAO Yu-dan, et al. Study on Content of Oleanolic Acid and Ursolic Acid in Different Processed Products and Different Years of Growth of Ligustri Lucidi Fructus[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(1): 46-48.
ZHANG Li, CHEN Ting, CAO Yu-dan, et al. Study on Content of Oleanolic Acid and Ursolic Acid in Different Processed Products and Different Years of Growth of Ligustri Lucidi Fructus[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(1): 46-48. DOI: 10.13422/j.cnki.syfjx.2011.01.026.
Study on Content of Oleanolic Acid and Ursolic Acid in Different Processed Products and Different Years of Growth of Ligustri Lucidi Fructus
Objective:To study on the content of oleanolic acid and ursolic acid in different processed products and different years of growth of Ligustri Lucidi Fructus by RP-HPLC.Method: Kromasil-C18 column(4.6 mm × 250 mm
5 μm) was used for the determination with the mobile phase consisting of [methanol-acetonitrile(50∶ 50)]-0.1% H3PO4 solution(85∶ 15);pH was adjusted to 6.5 with TEA;detection wavelength was at 205 nm;column temperature was set at 25 ℃ with the flow rate of 1 mL.min-1.Result: The content of oleanolic acid in different processed products was arranged in order of: steaming > scalding > steaming drugs with rice wine > crude drugs;the content of ursolic acid in different processed products was similar;the content of oleanolic acid and ursolic acid in different years of growth was arranged in order of: ten years > five years > two years.Conclusion: The content of oleanolic acid and ursolic acid in Ligustri Lucidi Fructus was related to the processing method and the year of growth.
Investigation of Factors Influencing on Extraction Efficiency of Active Constituents in Ligustri Lucidi Fructus and Optimization of Ethanol Extraction Process of Zhenzhi Granules
Optimization of Successive Ultrasound Countercurrent Extraction Technology of Ligustri Lucidi Fructus
Determination of Oleanolic Acid and Ursolic Acid in by HPLC
Determination of Oleanolic Acid and Ursolic Acid in by HPLC from Different Districts in Xinjiang
Related Author
Zhang Li
CHEN Ting
CAO Yu-dan
YAO Wei-feng
WANG Ying
CAO Wei
HE Jun-jie
CHEN Yan
Related Institution
Jiangxi College of Traditional Chinese Medicine
Dept.of Pharmacy,The First Affiliated Hospital of Xinjiang Medical University
Tianjin University of Traditional Chinese Medicine
Tianjin Insititute of Pharmaceutical
Anhui College of Traditional Chinese Medicine,Anhui Key Laboratory of Modernized Chinese Medicine