Determination of Luteolin in Cyperi Rhizoma and Cyperi Rhizoma Fried with Vinegar by High-performance Liquid Chromatography

LI Ying-xia1; LU Yong-hui1; FENG Wen2; XING Li-qin1

doi:10.13422/j.cnki.syfjx.2011.01.029

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Determination of Luteolin in Cyperi Rhizoma and Cyperi Rhizoma Fried with Vinegar by High-performance Liquid Chromatography

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- Determination of Luteolin in Cyperi Rhizoma and Cyperi Rhizoma Fried with Vinegar by High-performance Liquid Chromatography
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 1, Pages: 56-58(2011)
- 作者机构：
  
  1. 山东省中医药研究院
  2. 泰安市药品检验所
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2011.01.029
  CLC： R284.1
- Published：2011
- 稿件说明：
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[1]李英霞,陆永辉,冯文,邢丽芹.HPLC测定不同产地香附及醋炙香附中木犀草素的含量[J].中国实验方剂学杂志,2011,17(01):56-58.

LI Ying-xia1, LU Yong-hui1, FENG Wen2, et al. Determination of Luteolin in Cyperi Rhizoma and Cyperi Rhizoma Fried with Vinegar by High-performance Liquid Chromatography[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(1): 56-58.
[1]李英霞,陆永辉,冯文,邢丽芹.HPLC测定不同产地香附及醋炙香附中木犀草素的含量[J].中国实验方剂学杂志,2011,17(01):56-58. DOI： 10.13422/j.cnki.syfjx.2011.01.029.

LI Ying-xia1, LU Yong-hui1, FENG Wen2, et al. Determination of Luteolin in Cyperi Rhizoma and Cyperi Rhizoma Fried with Vinegar by High-performance Liquid Chromatography[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(1): 56-58. DOI： 10.13422/j.cnki.syfjx.2011.01.029.

摘要

目的:对不同产地香附及醋炙香附中木犀草素的含量进行测定。方法:采用高效液相色谱法

使用C18柱

以甲醇-0.2%磷酸水溶液（55∶45）为流动相

柱温30℃

流速1.0 mL.min-1

检测波长350 nm。结果:木犀草素在0.040 8～0.367 2μg线性关系良好

r=0.999 4。8个产地香附中木犀草素的含量以泰安产的最高

达57.35μg·g-1

除云南产的较低（17.82μg·g-1）外

其余产地均在22.00μg·g-1以上。香附炮制后木犀草素含量有不同程度的升高。结论:建立了HPLC测定香附及炮制品中木犀草素含量的测定方法

并探讨了香附醋炙前后木犀草素含量的变化

为探讨香附醋炙的科学内涵提供了一定参考。

Abstract

Objective:To determine the content of luteolin in Cyperi Rhizoma and Cyperi Rhizoma fried with vinegar from different areas.Method: The content was determined by HPLC and the measurement conditions were used as following: kromasil C18（4.6 mm × 150 mm

5 μm）;mobile phase: methanol-water with 0.2% phosphoric acid（55∶ 45）;the flow rate: 1.0 mL.min-1;measurement wavelength 350 nm.The temperature of column 30 ℃.Result: The linear range of luteolin was ranged from 0.040 81 to 0.367 2 μg（r = 0.999 4）.The content of luteo-lin was 57.35 μg·g-1 in Cyperi Rhizoma from Tai’an Shandong

which was the highest in 8 different areas

and 17.82 μg·g-1

the lowest from Yunnan

China.The content of luteolin from most areas exceeded 22.00 μg·g-1.The content of luteolin in Cyperi Rhizoma fried with vinegar from different areas was higher than these herbs before fring with vinegar.Conculsion: The study supplies a scientific basis for the content of luteolin in Cyperi Rhizoma and Cyperi Rhizoma fried with vinegar.

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