HPLC Determination of Hesperidin Content in Xiao’er Shenzhu Jianpi Pills

DENG Shuang-bing1; WANG Cheng-xia1; HU Yu-hua1; LI Jun-hong1; ZHANG Si-ju2

doi:10.13422/j.cnki.syfjx.2011.01.030

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HPLC Determination of Hesperidin Content in Xiao’er Shenzhu Jianpi Pills

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- HPLC Determination of Hesperidin Content in Xiao’er Shenzhu Jianpi Pills
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 1, Pages: 59-60(2011)
- 作者机构：
  
  1. 北京振东光明药物研究院有限公司国家中医药三级实验室
  2. 中国中医科学院中药研究所
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2011.01.030
  CLC： R286.0
- Published：2011
- 稿件说明：
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[1]邓双炳,王成霞,胡玉花,李军红,张思巨.HPLC测定小儿参术健脾丸中橙皮苷的含量[J].中国实验方剂学杂志,2011,17(01):59-60+63.

DENG Shuang-bing1, WANG Cheng-xia1, HU Yu-hua1, et al. HPLC Determination of Hesperidin Content in Xiao’er Shenzhu Jianpi Pills[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(1): 59-60.
[1]邓双炳,王成霞,胡玉花,李军红,张思巨.HPLC测定小儿参术健脾丸中橙皮苷的含量[J].中国实验方剂学杂志,2011,17(01):59-60+63. DOI： 10.13422/j.cnki.syfjx.2011.01.030.

DENG Shuang-bing1, WANG Cheng-xia1, HU Yu-hua1, et al. HPLC Determination of Hesperidin Content in Xiao’er Shenzhu Jianpi Pills[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(1): 59-60. DOI： 10.13422/j.cnki.syfjx.2011.01.030.

摘要

目的:为了提高小儿参术健脾丸的质量标准

建立小儿参术健脾丸中橙皮苷的含量测定方法。方法:采用高效液相色谱法

以甲醇-水（40:60）为流动相

色谱柱YMC-Pack ODS-A（4.6 mm×250 mm

5μm）

流速1.0 mL.min-1

检测波长283 nm

柱温35℃。结果:橙皮苷进样量在200.4～2004 ng与峰面积线性关系良好（r=0.999 9）;平均回收率为97.34%

RSD为1.27%。结论:本方法专属性强

准确、简便

可用于小儿参术健脾丸的质量控制。

Abstract

Objective:To improve the quality standard of Xiao’er Shenzhu Jianpi Pills

the study of HPLC methodology was carried out to determine the hesperidin in the Pills.Method: The separation was performed on YMC-Pack ODS-A（4.6 mm × 250 mm

5 μm）

using a mixture of methanol-water（40:60） as the mobile phase with the flow rate of 1.0 mL·min-1at 35 ℃;the detection wavelength was at 283 nm.Result: Hesperidin was linear within the range of 200.4-2004 ng（r = 0.999 9）.The average recovery for hesperidin was 97.34%

and RSD was 1.27%.Conclusion: The method was selective

accurate and simple

and suitable for controlling the quality of Xiao’er Shenzhu Jianpi Pills.

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Related Author

DENG Shuang-bing

WANG Cheng-xia

HU Yu-hua

LI Jun-hong

ZHANG Si-ju

Ya-wen WANG

Zhi-yun LIANG

Zhen-shan XIE

Related Institution

The First Clinical Medical College of Guangzhou University of Chinese Medicine

School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine

National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences

The First Affiliated Hospital of Guangzhou University of Chinese Medicine

National Insitutes for Food and Drug Control

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