Molecular Mechanism of Jianfei Capsule to Obese Rat behind Signal Transcription System

ZAI Jun-hua1; MENG Chang-hai1; DAI Xue-na1; CHENG Lu2; LI Qiang1; LIU Wei-hong1; LI Song-wei1; SHAO Ming-yi1; JIN Xiao-qin1

doi:10.13422/j.cnki.syfjx.2011.01.049

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Molecular Mechanism of Jianfei Capsule to Obese Rat behind Signal Transcription System

更新时间：2024-09-23

- Molecular Mechanism of Jianfei Capsule to Obese Rat behind Signal Transcription System
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 1, Pages: 130-133(2011)
- 作者机构：
  
  1. 河南中医学院第一附属医院
  2. 河南中医学院第三附属医院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2011.01.049
  CLC： R285.5
- Published：2011
- 稿件说明：
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[1]宰军华,孟长海,代雪娜,程璐,李强,刘卫红,李松伟,邵明义,金小琴.减肥胶囊对肥胖大鼠脂肪组织瘦素受体后JAK-STAT信号转录系统的影响[J].中国实验方剂学杂志,2011,17(01):130-133.

ZAI Jun-hua1, MENG Chang-hai1, DAI Xue-na1, et al. Molecular Mechanism of Jianfei Capsule to Obese Rat behind Signal Transcription System[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(1): 130-133.
[1]宰军华,孟长海,代雪娜,程璐,李强,刘卫红,李松伟,邵明义,金小琴.减肥胶囊对肥胖大鼠脂肪组织瘦素受体后JAK-STAT信号转录系统的影响[J].中国实验方剂学杂志,2011,17(01):130-133. DOI： 10.13422/j.cnki.syfjx.2011.01.049.

ZAI Jun-hua1, MENG Chang-hai1, DAI Xue-na1, et al. Molecular Mechanism of Jianfei Capsule to Obese Rat behind Signal Transcription System[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(1): 130-133. DOI： 10.13422/j.cnki.syfjx.2011.01.049.

摘要

目的:研究减肥胶囊对瘦素受体后信号转录系统的影响

从分子水平探讨减肥胶囊的减肥机制。方法:采用77只普通级SD雄性仔鼠

随机分为空白组10只和高脂饲料组67只

两组体重比较无显著性差异。空白组给予普通饲料

高脂饲料组给予高脂饲料

喂养8周后

选取高脂饲料组大鼠体重高于空白组大鼠平均体重的20%作为肥胖大鼠模型。将复制成功肥胖大鼠随机分为模型对照组、减肥胶囊高剂量组、低剂量组3组。各组均喂普通饲料

高剂量组和低剂量组分别按13.7

6.85 g·kg-1 ig减肥胶囊水煎液（10 mL·kg-1·d-1）

空白组和模型对照组ig等体积蒸馏水

共ig 4周。4周后

称重

麻醉大鼠

腹主动脉穿刺取血后

迅速摘取下丘脑和生殖器周围脂肪

采用组织原位杂交法检测下丘脑和脂肪组织酪氨酸蛋白激酶（JAK1）

信号转导及转录活化因子（STAT3）

信号转导及转录活化因子6（STAT6）基因表达水平。结果:不同浓度的减肥胶囊水煎液能够提升肥胖大鼠下丘脑和脂肪组织中JAK1

STAT3

STAT6基因表达水平

但同时发现

模型对照组下丘脑和脂肪组织中JAK1

STAT3

STAT6基因也处于高水平表达;高剂量组和低剂量组之间存在着一定的不统一性

存在着一定的量效关系。结论:减肥胶囊的作用机制可能是通过对下丘脑和脂肪组织中瘦素受体后信号转录系统的影响而发挥作用

但其机制还需要作进一步的探讨。

Abstract

Objective: To explore the influence of Jianfei capsule to leptin receptor（LR） behind signal transcription system and the mechanism at Molecular level.Method: The Seventy-seven male conventional Sprague-dawleg（SD） rats were divided into control diet group（n = ten） and high fat group（n = sixty-seven） randomly

there is no significant difference on the side of weight（P < 0.05）.And control diet group were fed with normal diet and high fat diet respectively.After eight weeks

thirty-five rats from high fat diet group were successfully made into obese models.Their body weight were all higher 20% than the average weight in control diet group.They were randomly divided into three groups.Obese model group

high dosage group and low dosage group.All of the groups were replaced by normal diet.Respectively by 13.7

6.85 g·kg-1

high and low dosage group were given Jianfei capsule decoction（10 mL·kg-1·d-1）

simultaneously

control group and obese model group were givenequivoluminal distilled water.After oral administration for four weeks

all of the rats were anaesthetized

weighted.After adopt blood from aorta abdominalisthe

Hypothalamic and genital fat

liver and heart were collected quickly.Gene expression of JAK1

STAT3

STAT6 of Hypothalamic and adipose tissue were detected by non-radioactive in situ-hybridization.Result:There are the high gene expression of JAK1

STAT3

STAT6 not only in Jianfei capsule but also in obese model group;there is some difference and dose-effect relationship between high dosage group with low dosage group.Third

different concentration Jianfei capsule can improve gene expression of JAK1

STAT3

STAT6 of hypothalamus.Conclusion:The mechanism that Jianfei capsule take effect through its influence to LR behind signal transcription system is still needed to be investigated further.

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Related Author

ZAI Jun-hua

MENG Chang-hai

DAI Xue-na

CHENG Lu

LI Qiang

LIU Wei-hong

LI Song-wei

SHAO Ming-yi

Related Institution

College of Basic Medical Sciences， Hubei University of Chinese Medicine

Laboratory Animal Center， Hubei University of Chinese Medicine

College of Traditional Chinese Medicine， Hubei University of Chinese Medicine

Hubei Shizhen Laboratory

First Clinical College，Hubei University of Chinese Medicine

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