TIAN Yun1, SONG Wen-ting2, XU Li2, et al. Effects of Nourishing Kidney Herbs on Dopamine of Striatal Neurons in Mouse Model of Parkinson ’s Disease[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(1): 134-137.
TIAN Yun1, SONG Wen-ting2, XU Li2, et al. Effects of Nourishing Kidney Herbs on Dopamine of Striatal Neurons in Mouse Model of Parkinson ’s Disease[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(1): 134-137. DOI: 10.13422/j.cnki.syfjx.2011.01.050.
Effects of Nourishing Kidney Herbs on Dopamine of Striatal Neurons in Mouse Model of Parkinson ’s Disease
Objective: To explore the effects of nourishing kidney herbs on dopamine of striatal neurons in mouse model of parkinson ’s disease.Method: Fourty five Parkinson’s disease model mice were randomly divided into five groups:Epimedii Folium group
Ligustri Lucidi Fructus group
Polygonatum group
selegiline group and model control group.Nine normal male C57BL /6J strains of mice were used as the normal control group.The normal control group and model control group were adminstraed with water
the mice in Epimedii Folium group
Ligustri Lucidi Fructus group
Polygonati Rhizoma group and selegiline group were adminstraed with Epimedii Folium
Ligustri Lucidum Fructus
Polygonatum and selegiline water decoction(each 0.5 mL
bid).After for 4 weeks
striatal neurons were quickly separated in the ice.Immunohistochemistry was used to detect the level of TH
HPLC-Electrochemical was used to detect the level of DA and metabolites.Result: The levels of TH
DA
DOPAC in Epimedii Folium group were significantly higher than that in model control group and selegiline group(P < 0.05)
were not significantly lower than that in the blank control group;The levels of DA were significantly higherin Ligustri lucidi Fructus group and Polygonati Rhizoma group than that in the model group(P < 0.05)
while the levels in Ligustrum lucidum group was higher than selegiline group(P < 0.05)
but not higher than that in the blank control group.Conclusion: The nourishing kidney herbs Epimedium Folium
Ligustri Lucidi Fructus and Polygonati Rhizoma can improve DA of striatal neurons in mouse model of parkinson ’s disease
but the mechanism is not exactly the same.Further study is needed.