Effect of Huangqin Decoction on CD4<sup>+</sup>T Cell and its Co-stimulator Factors for Unlcerative Colitis Rat with Damp-and-heat Syndrome

ZHENG Xue-bao1; FENG Yan-ling1; LIU Hong-bo1; DAI Shi-xue1

doi:10.13422/j.cnki.syfjx.2011.01.059

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Effect of Huangqin Decoction on CD4⁺T Cell and its Co-stimulator Factors for Unlcerative Colitis Rat with Damp-and-heat Syndrome

更新时间：2024-09-23

- Effect of Huangqin Decoction on CD4⁺T Cell and its Co-stimulator Factors for Unlcerative Colitis Rat with Damp-and-heat Syndrome
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 1, Pages: 169-172(2011)
- 作者机构：
  
  1. 广东医学院中医研究室
  2. 南方医科大学中医药学院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2011.01.059
  CLC： R285.5
- Published：2011
- 稿件说明：
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[1]郑学宝,封艳玲,刘洪波,戴世学.黄芩汤对湿热型溃疡性结肠炎大鼠CD4~+T细胞及其共刺激分子的影响[J].中国实验方剂学杂志,2011,17(01):169-172.

ZHENG Xue-bao1, FENG Yan-ling1, LIU Hong-bo1, et al. Effect of Huangqin Decoction on CD4⁺T Cell and its Co-stimulator Factors for Unlcerative Colitis Rat with Damp-and-heat Syndrome[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(1): 169-172.
[1]郑学宝,封艳玲,刘洪波,戴世学.黄芩汤对湿热型溃疡性结肠炎大鼠CD4~+T细胞及其共刺激分子的影响[J].中国实验方剂学杂志,2011,17(01):169-172. DOI： 10.13422/j.cnki.syfjx.2011.01.059.

ZHENG Xue-bao1, FENG Yan-ling1, LIU Hong-bo1, et al. Effect of Huangqin Decoction on CD4⁺T Cell and its Co-stimulator Factors for Unlcerative Colitis Rat with Damp-and-heat Syndrome[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(1): 169-172. DOI： 10.13422/j.cnki.syfjx.2011.01.059.

摘要

目的:观察CD4+T细胞及其共刺激分子（CSF）在湿热型溃疡性结肠炎（UC）大鼠肠黏膜中的表达及分布

探讨黄芩汤治疗该病可能的免疫学机制。方法:采用高脂、高糖饲料联合人工气候箱法造出湿热型体质

结合三硝基苯磺酸（TNBS）灌肠法建立湿热型UC模型;造模成功后将大鼠分为模型组、黄芩汤组及美沙拉嗪组

用免疫组化SP法检测大鼠结肠黏膜CD4+T细胞

CD28

CD152

OX40T细胞亚型的表达及分布

通过图像数码采集系统及Image pro plus 6.0软件分析平均光密度

以检测各分子表达含量。结果:与正常对照组比较

造模大鼠结肠中CD4+T细胞、CD28

CD152

OX40等T细胞亚型升高（P<0.05）

主要分布于黏膜下层及固有肌层;治疗后

黄芩汤和美沙拉嗪组大鼠结肠中CD4+T细胞

CD28

OX40T细胞亚型表达均下降（P<0.05）

但CD152的表达无明显变化且维持在模型组水平;黄芩汤组及美沙拉嗪组以上各分子的表达均无统计学差异。结论:黄芩汤可能通过调节CD4+T细胞的CSF的表达含量从而发挥对溃疡性结肠炎的免疫调节作用。

Abstract

Objective:To observe the expression and localization of CD4+T cell and its co-stimulator factors（CSF） in the mucosa of ulcerative colitis（UC）rat with damp-and-heat syndrome

and to explore the conceivable immunology mechanism of Huangqin decoction to treat the disease.Method:The model of UC rats with damp-andheat syndrome were established by giving the high sugar fat food and put into an artificial bioclimatic chamber

resulting in damp-and-heat body constitution and then clysmaed with 2

6-trinitro-benzene-sulfonic acid.After that the rats were divided into the model group

Huangqin decoction group and mesalazine group.SP of Immunohistochemistry was used to determine the expression and localization of CD4+T cell

CD28

CD152

OX40 T cell subsets in mucosa.The above antigens were tested using Image pro plus 6.0

an image acquisition system based on the digital camera and software

by analyzing their mean density.Result:Compared with the normal rats（n=15）

the expression of CD4 + T cell

CD28

CD152 and OX40 T cell subsets in mucosa increased for the model rats（n=38 in total and P<0.05）

and targeted to distribution in the submucosa and inherent layers.Compared with the model control group（n=12）

CD4+T cell

CD28 and OX40 in both Huangqin decoction（n=13） and mesalazine group（n=12） decreased（P<0.05）

but CD152 did not

kept in the level of model gruop.There was no statistical difference of the level of the above antigens between Huangqin decoction（n=13） and mesalazine group（n=12

P>0.05）.Conclusion: It is possible that the expression of the CSF for CD4+T cells maybe the immunology mechanism of the decotion to treat UC.

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Effect of Huangqin Decoction on CD4+T Cell and its Co-stimulator Factors for Unlcerative Colitis Rat with Damp-and-heat Syndrome

DOI：10.13422/j.cnki.syfjx.2011.01.059

摘要

Abstract

关键词

Keywords

references

Effect of Huangqin Decoction on CD4⁺T Cell and its Co-stimulator Factors for Unlcerative Colitis Rat with Damp-and-heat Syndrome