Analysis on Correlation of HPLC Fingerprint on Decoction Pieces and Decoction of Rhizoma Ligusticum

WEI Gang1; CAI Qing-qun2; HUANG Yue-chun2; LIU Cui-ling1

doi:10.13422/j.cnki.syfjx.2011.02.020

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Analysis on Correlation of HPLC Fingerprint on Decoction Pieces and Decoction of Rhizoma Ligusticum

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- Analysis on Correlation of HPLC Fingerprint on Decoction Pieces and Decoction of Rhizoma Ligusticum
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 2, Pages: 46-49(2011)
- 作者机构：
  
  1. 广州中医药大学
  2. 广州中医药大学第一附属医院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2011.02.020
  CLC： R284.1
- Published：2011
- 稿件说明：
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[1]魏刚,蔡庆群,黄月纯,刘翠玲.川芎饮片及其煎剂HPLC指纹图谱的相关性研究[J].中国实验方剂学杂志,2011,17(02):46-49.

WEI Gang1, CAI Qing-qun2, HUANG Yue-chun2, et al. Analysis on Correlation of HPLC Fingerprint on Decoction Pieces and Decoction of Rhizoma Ligusticum[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(2): 46-49.
[1]魏刚,蔡庆群,黄月纯,刘翠玲.川芎饮片及其煎剂HPLC指纹图谱的相关性研究[J].中国实验方剂学杂志,2011,17(02):46-49. DOI： 10.13422/j.cnki.syfjx.2011.02.020.

WEI Gang1, CAI Qing-qun2, HUANG Yue-chun2, et al. Analysis on Correlation of HPLC Fingerprint on Decoction Pieces and Decoction of Rhizoma Ligusticum[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(2): 46-49. DOI： 10.13422/j.cnki.syfjx.2011.02.020.

摘要

目的:研究川芎饮片及其煎剂指纹图谱剂的相关性。方法:采用HPLC分析指纹图谱

色谱柱Zorbax SB-Aq;流动相乙腈-0.05%磷酸溶液（梯度洗脱）;检测波长290 nm;流速1 mL·min-1;柱温35℃。结果:川芎饮片标示出20个主要特征峰

10批样品的相似度在0.821～0.994

有两批样品相似度小于0.9。川芎煎剂能检出川芎的16个主要特征峰（峰1～16）

10批煎剂的相似度在0.958～0.998

相似度较高。川芎煎剂的16个特征峰面积的提取率在10.67%～111.63%

差异相当大

极性大的成分提取率明显较高

极性小的成分提取率明显较低。饮片与煎剂之间的相似度为0.481～0.970

有两批样品相似度大于0.9。结论:传统煎煮法制备的川芎煎剂基本能检出川芎的大部分特征峰

但特征峰面积的相对比值差异较大

本研究为川芎、川芎煎剂、含川芎复方煎剂的指纹图谱研究提供了一定参考。

Abstract

Objective:To study the correlation of HPLC fingerprint on the decoction pieces and decoction of Rhizoma Ligusticum.Method:HPLC with Zorbax SB-Aq was used

acetonitrile-0.05% phosphoric acid（gradient elution）as a mobile phase

detection wavelength was at 290 nm

flow rate was 1 mL.min-1

and column temperature was 35 ℃.Result: Twenty common peaks were separated in the decoction pieces of Rhizoma Ligusticum

the similarity of 10 samples was 0.821-0.994.16 common peaks（pesk 1-pesk 16） were separated in the decoction of Rhizoma Ligusticum

the similarity of 10 samples was 0.958-0.998.The extraction percentages of 16 peaks of the decoction were 10.67%-111.63%

there were larger difference.The similarity between the decoction pieces and decoction was 0.481-0.970.Conclusion: The much composition of Rhizoma Ligusticum could be extracted by traditional decoction method.The results can provide a reference for the study of the fingerprint of Rhizoma Ligusticum

Rhizoma Ligusticum decoction or preparations containing Rhizoma Ligusticum.

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Related Institution

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