FU Ke, WANG Jian-gong, YAN Guang-li. Determination of Saikosaponin A in Chaiqin Qinggan Formula Extract by HPLC-ELSD[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(2): 57-59.
FU Ke, WANG Jian-gong, YAN Guang-li. Determination of Saikosaponin A in Chaiqin Qinggan Formula Extract by HPLC-ELSD[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(2): 57-59. DOI: 10.13422/j.cnki.syfjx.2011.02.022.
Determination of Saikosaponin A in Chaiqin Qinggan Formula Extract by HPLC-ELSD
摘要
目的:建立柴芩清肝方提取物中柴胡皂苷a的含量测定方法。方法:采用Diamonsil C18柱(4.6 mm×200 mm
Objective:To establish a HPLC method for the determination of saikosaponin A in the extract of Chaiqin Qinggan formula.Method:The column of Diamonsil C18(4.6 mm×200 mm
5 μm) was used.The mobile phase was acetonitrile-water with a gradient elution program with the flow rate at 1.0 mL·min-1.The evaporative light scattering detector(ELSD) was used as detector.Drift tube temperature was at 105℃
and the gas flow rate was 2.0 L·min-1.Result:The logarithmic calibration curves were linear in the range of 1.7-8.5 μg(r=0.999 4).The average recovery was 96.6% with the RSD value of 1.8%(n = 5).Conclusion:The method can be used to determine the content of saikosaponin a in the extract of Chaiqin Qinggan formula.