Simultaneous Determination of Chlorogenic Acid and Galuteolin in Honeysuckle

XIN Hua1; FENG Jie2; CHENG Ruo-min1; XIN Ning1

doi:10.13422/j.cnki.syfjx.2011.02.023

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Simultaneous Determination of Chlorogenic Acid and Galuteolin in Honeysuckle

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- Simultaneous Determination of Chlorogenic Acid and Galuteolin in Honeysuckle
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 2, Pages: 60-63(2011)
- 作者机构：
  
  1. 广西中医学院
  2. 中国科学院理化技术研究所
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2011.02.023
  CLC： R284
- Published：2011
- 稿件说明：
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[1]辛华,丰杰,程若敏,辛宁.HPLC测定不同产地金银花中绿原酸和木犀草苷[J].中国实验方剂学杂志,2011,17(02):60-63.

XIN Hua1, FENG Jie2, CHENG Ruo-min1, et al. Simultaneous Determination of Chlorogenic Acid and Galuteolin in Honeysuckle[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(2): 60-63.
[1]辛华,丰杰,程若敏,辛宁.HPLC测定不同产地金银花中绿原酸和木犀草苷[J].中国实验方剂学杂志,2011,17(02):60-63. DOI： 10.13422/j.cnki.syfjx.2011.02.023.

XIN Hua1, FENG Jie2, CHENG Ruo-min1, et al. Simultaneous Determination of Chlorogenic Acid and Galuteolin in Honeysuckle[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(2): 60-63. DOI： 10.13422/j.cnki.syfjx.2011.02.023.

摘要

目的:建立高效液相转换波长法

同时测定金银花中绿原酸和木犀草苷的含量。方法:采用Hypersil gold C18色谱柱（4.6 mm×250 mm

5μm）

乙腈-0.05%磷酸水溶液梯度洗脱;流速0.5 mL·min-1

柱温25℃

检测波长327 nm

350 nm。结果:绿原酸和木犀草苷的质量浓度分别在0.06×103～0.42×103 mg·L-1（r=0.999 9）和0.716～5.728 mg·L-1（r=0.999 95）与峰面积呈良好的线性关系（n=5）

平均回收率（n=6）分别为101.15%和99.80%。结论:该方法同时测定金银花中的绿原酸和木犀草苷的含量

结果准确度高

精密度好

可为金银花药材的质量控制研究提供一种可靠的参考方法。

Abstract

Objective:To establish a wavelength conversion method for determination of chlorogenic acid and galuteolin in honeysuckle simultaneously.Method:The separation was performed on a Hypersil gold C18 column （4.6 mm × 250 mm

5 μm）.Acetonitril-0.05% H3P04 was used as the mobile phase with gradient elution.The flow rate was 0.5 mL·min-1.The detection wavelength was at 327 nm and 350 nm.Result:The linear range（n=5）was 0.06 × 103-0.42 × 103 mg·L-1（r=0.999 9） and 0.716-5.728 g·L-1（r=0.999 95） respectively.The average recoveries（n=6） of chlorogenic acid and galuteolin were 101.15% and 99.80% respectively.Conclusion:This method is sensitive

reliable and can be applied to determine chlorogenic acid and galuteolin in the raw material of honeysuckle.

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Related Institution

Institute of Traditional Chinese Medicine（TCM），Shanghai University of TCM

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Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

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