Determination of Psoralen in Ficus Leaves

WANG Hong-xia; ZHENG Yan; CHEN Sui-qing; ZHU Xiao-cong; ZHANG Fei

doi:10.13422/j.cnki.syfjx.2011.02.024

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Determination of Psoralen in Ficus Leaves

更新时间：2024-09-23

- Determination of Psoralen in Ficus Leaves
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 2, Pages: 64-67(2011)
- 作者机构：
  
  河南中医学院药学院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2011.02.024
  CLC： R284.1
- Published：2011
- 稿件说明：
移动端阅览
[1]王红霞,郑岩,陈随清,朱晓丛,张飞.无花果叶的质量分析[J].中国实验方剂学杂志,2011,17(02):64-67.

WANG Hong-xia, ZHENG Yan, CHEN Sui-qing, et al. Determination of Psoralen in Ficus Leaves[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(2): 64-67.
[1]王红霞,郑岩,陈随清,朱晓丛,张飞.无花果叶的质量分析[J].中国实验方剂学杂志,2011,17(02):64-67. DOI： 10.13422/j.cnki.syfjx.2011.02.024.

WANG Hong-xia, ZHENG Yan, CHEN Sui-qing, et al. Determination of Psoralen in Ficus Leaves[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(2): 64-67. DOI： 10.13422/j.cnki.syfjx.2011.02.024.

摘要

目的:通过对无花果叶的定性、定量分析研究

为无花果叶的开发利用、质量标准的制定提供依据。方法:运用薄层色谱、紫外光谱法对无花果叶进行定性理化特征鉴定

以补骨脂素、佛手柑内酯为对照品

运用HPLC对无花果叶进行含量测定。结果:薄层色谱法鉴别色谱斑点清晰

专属性强;无花果叶主成分在290 nm处紫外吸收最大;补骨脂素在0.083 2～0.832μg线性关系良好

回收率97.0%～104.1%

RSD 2.80%;佛手柑内酯在0.038 4～0.230 4μg线性关系良好

回收率在97.1%～103.4%

RSD 2.19%。结论:本文研究结果可作为无花果叶质量标准制定提供定性、定量方面的依据。

Abstract

Objective:The research provided reference for the exploitation and quantity control of Ficus leaves.by determinating quality and quantity analysis of Ficus leaves.Method:Determine the qualitative analysis of Ficus leaves by TLC and UV and determinate the quantitiy of psoralen and bergapten range by HPLC.Result: Between 0.083 2-0.832 μg psoralen has a good linearity

recoveries between the 97.0%-104.1%

and RSD is 2.80%;bergapten range between 0.038 4-0.230 4 μg has a good linearity

recoveries between 97.1%-103.4%

RSD is 2.19%.Under the conditions of two ingredients stability

reproducibility and precision are good.Conclusion:The study result can be used to provide basis to determine the quality control of Ficus leaves.

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Related Author

WANG Hong-xia

ZHENG Yan

CHEN Sui-qing

ZHU Xiao-cong

ZHANG Fei

ZHANG Xiao-yan

YANG Xiu-ping

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Related Institution

1*),LIU Zi-dong~1

第四军医大学西京医院药剂科

Pharmaceutical College,Jilin University

Jilin Agricultural University

Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences

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