Determination of Ginsenoside Rb<sub>1</sub> in Lianli Tang by HPLC Method

WANG Chun; SONG Zhi-qian; LI Qing; XIA Lei; WANG Chao; LIU Zhen-li

doi:10.13422/j.cnki.syfjx.2011.02.025

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Determination of Ginsenoside Rb₁ in Lianli Tang by HPLC Method

更新时间：2024-09-23

- Determination of Ginsenoside Rb₁ in Lianli Tang by HPLC Method
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 2, Pages: 68-69(2011)
- 作者机构：
  
  中国中医科学院中医基础理论研究所
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2011.02.025
  CLC： R284.1
- Published：2011
- 稿件说明：
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[1]王淳,宋志前,李青,夏磊,王超,刘振丽.连理汤中人参皂苷Rb_1含量测定[J].中国实验方剂学杂志,2011,17(02):68-69.

WANG Chun, SONG Zhi-qian, LI Qing, et al. Determination of Ginsenoside Rb₁ in Lianli Tang by HPLC Method[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(2): 68-69.
[1]王淳,宋志前,李青,夏磊,王超,刘振丽.连理汤中人参皂苷Rb_1含量测定[J].中国实验方剂学杂志,2011,17(02):68-69. DOI： 10.13422/j.cnki.syfjx.2011.02.025.

WANG Chun, SONG Zhi-qian, LI Qing, et al. Determination of Ginsenoside Rb₁ in Lianli Tang by HPLC Method[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(2): 68-69. DOI： 10.13422/j.cnki.syfjx.2011.02.025.

摘要

目的:建立高效液相色谱法（HPLC）测定连理汤中人参皂苷Rb1含量的方法。方法:采用Zobax Extend-C18（4.6 mm×150 mm

5μm）色谱柱

流动相乙腈-水（25∶75）;检测波长203 nm;流速1.0 mL·min-1;柱温40℃。结果:两个批次连理汤样品中人参皂苷Rb1含量分别为1.58 mg.g-1和1.47 mg·g-1。结论:方法稳定可靠

重复性好

可用于连理汤中人参皂苷Rb1的含量测定并控制制剂的质量。

Abstract

Objective:To develop a HPLC method for the determination of Ginsenoside Rb1 in Lianli Tang.Method:The HPLC method was applied with Agilent Zobax Extend-C18 colunmn by acetonitrile-water（25∶75） as the mobile phase

The flow rate was 1.0 mL·min-1.The column temperature was 40℃ and the UV detection wavelength was 203 nm.Result:The contents of ginsenoside Rb1 in two batch samples are 1.58 mg·g-1 and 1.47 mg·g-1.Conclusion:The method is stable and reliable

and can be used to assay ginsenoside Rb1 then control the quality of Lianli tang.

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WANG Chun

SONG Zhi-qian

LI Qing

XIA Lei

WANG Chao

LIU Zhen-li

LI Yu-cui

CHEN Zhi-wei

Related Institution

Nanjing University of Chinese Medicine

Jiangsu Key Laboratory for Traditional Chinese Medicine Formulae Research

Department of Pharmacy,Dali University,Dali

Shandong Academy of Traditional Chinese Medicine

Taian Institute for Drug Control,Tai′an

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Determination of Ginsenoside Rb1 in Lianli Tang by HPLC Method

DOI：10.13422/j.cnki.syfjx.2011.02.025

摘要

Abstract

关键词

Keywords

references

Determination of Ginsenoside Rb₁ in Lianli Tang by HPLC Method